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About This Item
UNSPSC Code:
41106514
Biological source:
human skin
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Not specified
biological source
human skin
packaging
tube of 5 μg 96020925-DNA-5UG, pkg of vial of cells 96020925-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human Amelanotic Melanoma
This cell line has been derived by the selection of the human amelanotic melanoma cell line C32 for 6-thioguanine resistance. The parent cell line C32 was established from a melanoma from a 53 year old Caucasian male. The cells are resistant to 6-thioguanine and sensitive to HAT medium. They are deficient in hypoxanthine ? guanine phosphoribosyl transferase (HGPRT-). The cell line is also known as C32-r16TG. The cells are tumorigenic in nude mice inoculated subcutaneously with 10E7 cells.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.050mM 6-thioguanine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80 %) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37 C; 5% CO2.
Other Notes
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Regulatory Information
低风险生物材料
常规特殊物品
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