C32TG

Human Amelanotic Melanoma

This cell line has been derived by the selection of the human amelanotic melanoma cell line C32 for 6-thioguanine resistance. The parent cell line C32 was established from a melanoma from a 53 year old Caucasian male. The cells are resistant to 6-thioguanine and sensitive to HAT medium. They are deficient in hypoxanthine ? guanine phosphoribosyl transferase (HGPRT-). The cell line is also known as C32-r16TG. The cells are tumorigenic in nude mice inoculated subcutaneously with 10E7 cells.

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.050mM 6-thioguanine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80 %) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 37 C; 5% CO₂.

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.