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About This Item
UNSPSC Code:
41106514
Biological source:
human lung (adrenal metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent/aggregates in suspension
Karyotype:
Hypotriploid; modal number = 54; range = 46 to 57. Twenty to 25 marker chromosomes common to all cells. These include t(13q21q), der(1)t(1;21)(p36.1;q11), der(7)t(7;7)(p22;q22), 11p+, 12p+, and 15p+.
Morphology:
Not specified
biological source
human lung (adrenal metastasis)
packaging
tube of 5 μg 96020944-DNA-5UG, pkg of vial of cells 96020944-1VL
growth mode
Adherent/aggregates in suspension
karyotype
Hypotriploid; modal number = 54; range = 46 to 57. Twenty to 25 marker chromosomes common to all cells. These include t(13q21q), der(1)t(1;21)(p36.1;q11), der(7)t(7;7)(p22;q22), 11p+, 12p+, and 15p+.
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human lung carcinoma, adrenal gland metastasis
The NCI-H510A cell line was derived by D. Carney, A.F. Gazdar amd associates in 1982 from an adrenal metastasis in an adult, 56-year-old, male Caucasian patient. The cells produce easily detectable p53 mRNA at levels comparable to those in normal lung tissue. The line does not exhibit any gross structural DNA abnormalities. The cells express elevated levels of four biochemical markers of small cell lung carcinoma (SCLC) i.e. neuron specific enolase, the brain isoenzyme creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. This cell line is tumourigenic in nude mice (the cells form transplantable tumours with typical small cell carcinoma histology).
Preparation Note
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 2-4x10,000 cells/cm2; 5% CO2; 37°C. This cell line grows as a mixture of cells in suspension and adherent cells. Subcultures can be prepared by scraping the adherent cells into the medium,
Other Notes
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Regulatory Information
低风险生物材料
常规特殊物品
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