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Merck
CN

ChaGo-K-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96020948, human lung (bronchus), Epithelial

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About This Item

UNSPSC Code:
41106514
Biological source:
human lung (bronchus)
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Epithelial
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biological source

human lung (bronchus)

packaging

tube of 5 μg 96020948-DNA-5UG, pkg of vial of cells 96020948-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Biochem/physiol Actions

Human lung bronchus carcinoma
This cell line was derived from a bronchogenic carcinoma of a 45-year-old male. The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.

Preparation Note

RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

低风险生物材料
常规特殊物品
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