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Merck
CN

MCP21

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC Code:
41106514
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biological source

Mouse x Mouse Hybridoma

packaging

tube of 5 μg 96030418-DNA-5UG
pkg of vial of cells 96030418-1VL

ImmunoDon

BALB/c mouse spleen

Myeloma

Sp2/0

Reactivity

Anti-Proteasomes, human

technique(s)

cell culture | mammalian: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−196°C

Immunogen

Human placenta proteasomes

Application

Detection and purification of proteasomes and analysis of subunits

Biochem/physiol Actions

Antibodies secreted by the hybridoma line MCP21 react with subunit C3 in human and rabbit proteasomes, both with intact proteasomes and on blots. The hybridoma has been produced by repeated intraperitoneal immunisation of BALB/c mice with 20-50 μg of human placenta proteasomes and subsequent fusion of spleen cells with myeloma Sp2/0.
Mouse Sp2/0 x Mouse BALB/c spleen

Preparation Note

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Maintain cultures between 5x10,000 and 1x1,000,000 cells/ml; 5% CO2; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Regulatory Information

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