PEA-10

Study of LRP and LRP deficiency.

Mouse embryo fibroblast, LRP-deficient, heterozygous

The cell line PEA-10 is heterozygous for a disruption of the gene for low density lipoprotein receptor-related protein (LRP). PEA-10 was derived from fibroblasts from day 15 C57BL/6x129 F1 hybrid mouse embryos. Together with the cell lines PEA-13 (Sigma Catalogue number. 97040311) and MEF-1 (Sigma Catalogue number. 98061101) an experimental system is available for the analysis of cellular uptake of functionally diverse ligands and the effect of LRP deficiency. This is of importance since LRP deficient mouse embryos die early during gestation.

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split confleunt cultures 1:6 to 1:10 i.e. seeding 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.