biological source
human breast
packaging
tube of 5 μg 98050130-DNA-5UG
pkg of vial of cells 98050130-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Androgen receptor
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Androgen receptor studies, study of mammary carcinoma
Biochem/physiol Actions
Human caucasian mammary carcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 11,12
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 17
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 11,12
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 17
The mammary carcinoma cell line MFM-223 was obtained from the pleural effusion of a postmenopausal breast cancer patient. The patient had not received any prior treatment and the tumour was classified as a widespread ductal mammary carcinoma with the histological grade of III. The cell line is characterised by high androgen, (approximately 100,000 binding sites per cell) and low oestrogen and progesterone receptor levels. Proliferation of cells was inhibited by addition of the androgen dihydrotestosterone at concentrations above 0.01 nM. MFM-223 have a characteristic pattern of isoenzyme polymorphism and of karyotype alterations revealing marker chromosomes and homogenously staining regions. Most cells have been near diploid with 10% displaying near tetraploidy. The cells have been tested to be free of Hepatitis B and C viruses and HIV. MFM-223 is tumourigenic in nude mice.
Preparation Note
MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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