GM1899A Human Not specified, ECACC 98120701

Feeder for T-lymphocyte culture

Human lymphoblast, HPRT - deficient

The lymphoblastoid cell line GM1899A has been derived from a Lesch-Nyhan patient. It is deficient for hypoxanthine-guanine phosphoribosyl transferase (HPRT) with a point mutation in the respective gene. This cell line is used as a feeder for extended-term culture of human T-lymphocytes (see LB lines in the collection).

Dutch Modified RPMI 1640 + 2mM Glutamine + 20mM Hepes + 2mM Sodium Pyruvate (NaPy) + 20% Heated Inactivated Foetal Bovine Serum (HI-FBS)

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. Do not dilute below 1 x 100,000 cells/ml, wash 2-3 times after resuscitation before seeding; disaggregate every few days by pipetting to avoid formation of large aggregates

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.