Product Name
Sepharose™ CL-6B, Cross-linked
form
suspension
bead diameter
40-165 μm
pore size
10,000-1,000,000 fractionation range (Dextrans)
10,000-4,000,000 fractionation range (Globular proteins)
storage temp.
2-8°C
Quality Level
Related Categories
Application
Sepharose™CL-6B has been used for the purification of glucoamylase enzyme and targeted ribonucleoprotein (RNP).
Sepharose CL-6B is used in affinity chromatography, protein chromatography, gel filtration chromatography, separation media and resins. Sepharose™ CL-6B has been used to study in vitro inhibition of human melanoma cells and to gather data in other valuable anticancer studies.
General description
Sepharose CL is a cross-linked derivative of sepharose, prepared by reacting sepharose with 2,3-dibromopropanol under strongly alkaline conditions.
Legal Information
Sepharose is a trademark of Cytiva
signalword
Danger
hcodes
Hazard Classifications
Flam. Liq. 2
Storage Class
3 - Flammable liquids
wgk
WGK 3
Regulatory Information
危险化学品
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M Schweizer et al.
Molecular & general genetics : MGG, 203(3), 479-486 (1986-06-01)
FAS1, the structural gene of the pentafunctional fatty acid synthetase subunit beta in Saccharomyces cerevisiae has been sequenced. Its reading frame represents an intron-free nucleotide sequence of 5,535 base pairs, corresponding to a protein of 1,845 amino acids with a
W Chen et al.
Journal of bacteriology, 175(17), 5677-5682 (1993-09-01)
A membrane-associated ATPase with an M(r) of approximately 510,000 and containing subunits with M(r)s of 80,000 (alpha), 55,000 (beta), and 25,000 (gamma) was isolated from the methanogen Methanococcus voltae. Enzymatic activity was not affected by vanadate or azide, inhibitors of
Ru-Ping Zhang et al.
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 30(6), 561-564 (2002-08-09)
Luffin B, a plan single-chain ribosome inactivating protein, was purified from seeds of Luffa cylindrica by Blue Sepharose CL-6B affinity chromatography. An immunotoxin was constructed with luffin B and Ng76, a monoclonal antibody to human melanoma cell M(21). Luffin B-Ng76
C C Chadwick et al.
Archives of biochemistry and biophysics, 252(2), 348-356 (1987-02-01)
As isolated by our recently developed large-scale procedure, the Neurospora plasma membrane H+-ATPase exists as a homogeneous, oligomeric complex of 105,000-Da monomers with a molecular mass equivalent to a spherical protein of about 1 million Da, as judged by its
Amylolytic fungi in starter cakes for rice beer production
Das AJ, et al.
The Journal of General and Applied Microbiology, 63(4), 236-245 (2017)
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