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Merck
CN

D2532

Sigma-Aldrich

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

Synonym(s):

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

Key Documents

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Quality Level

300

form

solution

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

Related Categories

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Preparation Note

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品

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Certificates of Analysis (COA)

Lot/Batch Number
0000411631
0000411631
0000375543
SLCN0620
0000320236

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It has long been a question whether Staphylococcus aureus, a major human pathogen, is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary sigma factor, SigH, was a likely key component for
M A Hull et al.
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J Stokes et al.
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Protocols

Ficoll® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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