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E3024

Earle′s Balanced Salts

With sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

EBSS

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217343
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Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
NaHCO3: 2.2 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

storage temp.

room temp

General description

Earle′s balanced salts solution (EBSS) is a saline solution with physiological pH that stimulates autophagy. It augments Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) by upregulating cluster of differentiation (CD86), human leukocyte antigen-DR isotype (HLA-DR), and interleukin (IL-6).

Application

Earle′s balanced salts has been used to incubate filter-grown monolayers of Caco-2 cells to induce starvation, along with HEPES buffer to detect NADPH oxidase activity in trypsinized cells. It has also been used to treat CEM RES cells and perform amino acid depletion experiment.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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