≥99% (HPLC)
powder
white
282-286 °C (dec.) (lit.)
1 M NH4OH: soluble
DMSO/DMF: soluble
methanol: soluble
7.07 at 265 nm in 0.1 M HCl
diagnostic assay manufacturing
hematology
histology
room temp
FC1=CNC(=O)NC1=O
1S/C4H3FN2O2/c5-2-1-6-4(9)7-3(2)8/h1H,(H2,6,7,8,9)
GHASVSINZRGABV-UHFFFAOYSA-N
human ... TYMS(7298)
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Danger
Acute Tox. 3 Oral - Carc. 2
6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK 3
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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No. No sterility or endotoxin testing has been performed. Solutions of the product can be sterile filtered before use.
The purity of Product No. F6627 is determined by TLC (thin layer chromatography). The purity of deleted product 47576 was determined by HPLC (high performance liquid chromatography).
Although product F6627 is not tested for solubility in water, there should be some limited solubility. Product 47576 has been tested at 10 mg/mL in water, although heat had to be applied. Upon cooling it might be possible to observe some precipitation. No testing in water is performed at Sigma on a lot to lot basis. The product is more soluble at higher alkaline pH.
Some cells, and in particular cancer cells, may be resistant to 5-fluorouracil. Please see this paper Huanwen, W., et al., Molecular Cancer, 8, 125 (2009) which addresses this observation, as well as references 16 and 30 in this publication.If the cells are treated with the closely related gemcitabine (Sigma-Aldrich Product G6423), and cell growth is not inhibited by that drug either, you will have shown that the "problem" is with the cell line, and not with the drug(s).
Sigma-Aldrich lists several fluorouracil products, but we do not offer Fluorouracil for injection. Our 5-Fluorouracil (5-FU) products cannot be given to human patients. (Note that although Product F8423 meets USP testing specifications, it is not a pharmaceutical grade or PharmaGrade product.)Having said that, a solution for injection into research animals could be prepared the same way as the Fluorouracil Injection, USP is. According to the drugs.com, "Fluorouracil Injection, USP, an antineoplastic anti-metabolite, is a colorless to yellow aqueous sterile, nonpyrogenic injectable solution for intravenous administration. Each mL contains: 50 mg fluorouracil; pH is adjusted to 8.6 to 9.4 with sodium hydroxide".
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