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G1544

Anti-GFP, N-terminal antibody produced in rabbit

Name: Anti-GFP, N-terminal antibody produced in rabbit
Brand: SIGMA

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Green Fluorescent Protein

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.56

MDL number:

MFCD05665217

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IP, WB

Citations:

300

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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates, western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

Primary Antibodies

General description

Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.

Immunogen

synthetic peptide corresponding amino acids 3-17 of GFP.

Application

Anti-GFP, N-terminal antibody produced in rabbit has been used:

in immunoblotting
in the histochemistry and immunohistochemistry
in the immunoprecipitation

Biochem/physiol Actions

Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.

Specific for GFP fusion proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

常规特殊物品

低风险生物材料

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Certificates of Analysis (COA)

Lot/Batch Number

0000425859

0000382649

0000350272

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Notch signaling controls the differentiation of transporting epithelia and multiciliated cells in the zebrafish pronephros

Liu Y, et al.

Development, 134(6), 1111-1122 (2007)

Green fluorescent protein: a perspective

Remington SJ

Protein Science, 20(9), 1509-1519 (2011)

A phosphorylation-wide sncRNA screen reveals Protein Functional Effector sncRNAs (pfeRNAs) in human lung somatic cells

Gable T, et al.

Cancer letters, 396(6), 85-93 (2017)

Production and histological application of affinity-purified antibodies to heat-denatured green fluorescent protein

Nakamura KC, et al.

The Journal of Histochemistry and Cytochemistry, 56(7), 647-657 (2008)

The Cell Death Pathway Regulates Synapse Elimination through Cleavage of Gelsolin in Caenorhabditis elegans Neurons.

Lingfeng Meng et al.

Cell reports, 11(11), 1737-1748 (2015-06-16)

Synapse elimination occurs in development, plasticity, and disease. Although the importance of synapse elimination has been documented in many studies, the molecular mechanisms underlying this process are unclear. Here, using the development of C. elegans RME neurons as a model

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Global Trade Item Number

SKU	GTIN
G1544-100UG	04061833628270

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