usage
sufficient for 4 96-well plate purifications
quality
4 x 96 well plate
storage temp.
15-25°C
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General description
GenElute 96 Well Tissue Genomic DNA Purification Kit allows for high throughput purification of pure genomic DNA from a variety of cultured cells and tissues. The kit contains the advantages of silica membrane technology and eliminates the need for expensive resins, alcohol precipitations, and hazardous compounds such as phenol and chloroform.
The starting materials are lysed in a solution containing SDS and Proteinase K. Once lysis is complete, chaotropic salts are added to further promote solubilization of macromolecules. The next step is to add ethanol which ensures that the appropriate binding conditions are created to bind the DNA to the silica membrane. Once the DNA is bound to the silica membrane several wash buffers are used to remove contaminants. The final step is to elute pure genomic DNA in the Tissue Elution Buffer included in the kit.
The purified genomic DNA is ready for use in downstream applications such as PCR, Southern blotting, or any kind of enzymatic reaction.
The starting materials are lysed in a solution containing SDS and Proteinase K. Once lysis is complete, chaotropic salts are added to further promote solubilization of macromolecules. The next step is to add ethanol which ensures that the appropriate binding conditions are created to bind the DNA to the silica membrane. Once the DNA is bound to the silica membrane several wash buffers are used to remove contaminants. The final step is to elute pure genomic DNA in the Tissue Elution Buffer included in the kit.
The purified genomic DNA is ready for use in downstream applications such as PCR, Southern blotting, or any kind of enzymatic reaction.
Application
The purified genomic DNA is ready for use in downstream applications such as:
- PCR
- Southern blots
- cloning
- restriction digest
- enzymatic reactions
Features and Benefits
- Starting material: ≤ 20 mg tissue total (mouse, rat or whole sample) or ≤ 1 X 106 cultured cells or bacteria per well
- Average Expected yield: 10 - 20 μg/well (bacteria: 1.5 - 2.5 μg/well)
- Elution volume: 100 μl
- Time required: 70 min/plate
- Innovative wash plate minimizes risk of cross-contamination
- Processing possible under vacuum or centrifugation
- Suitable for manual and automated processing
Principle
The starting materials are lysed in a solution containing SDS and Proteinase K. Once lysis is complete, chaotropic salts are added to further promote solubilization of macromolecules. The next step is to add ethanol, which ensures that the appropriate binding conditions are created to bind the DNA to the silica membrane. Once the DNA is bound to the silica membrane, several wash buffers are used to remove contaminants. The final step is to elute pure genomic DNA in the Tissue Elution Buffer included in the kit.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system, Respiratory system
Storage Class Code
3 - Flammable liquids
Flash Point(F)
75.2 °F
Flash Point(C)
24 °C
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