Quality Segment
technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 12-15 mL gel
bead diameter
40-120 μm
application(s)
life science and biopharma
compatibility
Cytiva
General description
Sephadex G-75 is widely used for desalting and buffer exchange. This gel filtration resin is created through the crosslinking of dextran with epichlorohydrin. Sephadex varies in its cross-linking level across different types, influencing its swelling capacity and molecular fractionation range.
Sephadex® G-75 is a gel filtration media used in gel filtration chromatography and protein chromatography. Sephadex® G-75 has been used to study cystatin of the mammalian brain, suggest candidates for development of novel oral or other anti-infective agents and to purify procoagulant metalloproteinase from rattlesnake venom.
Application
Sephadex® G-75 has been used to purify low density lipoproteins (LDL) by gel filtration.
Sephadex® G-75 has been used to separate the products from the pepsin digest by gel filtration chromatography and to desalt low density lipoproteins (LDL) by gel filtration.
Features and Benefits
- Enables rapid desalting, contaminant removal, and buffer exchange in a single step.
- Ideal for buffer exchange and cleanup of large biomolecules with a molecular weight exceeding 80,000.
- Features the highest exclusion limit of 80,000 within the Sephadex G-range.
- Classic gel filtration resin.
Legal Information
Sephadex is a registered trademark of Cytiva
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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