Quality Level
technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 12-15 mL gel
bead diameter
40-120 μm
application(s)
life science and biopharma
compatibility
Cytiva
General description
Sephadex G-75 is widely used for desalting and buffer exchange. This gel filtration resin is created through the crosslinking of dextran with epichlorohydrin. Sephadex varies in its cross-linking level across different types, influencing its swelling capacity and molecular fractionation range.
Sephadex® G-75 is a gel filtration media used in gel filtration chromatography and protein chromatography. Sephadex® G-75 has been used to study cystatin of the mammalian brain, suggest candidates for development of novel oral or other anti-infective agents and to purify procoagulant metalloproteinase from rattlesnake venom.
Application
Sephadex® G-75 has been used to purify low density lipoproteins (LDL) by gel filtration.
Sephadex® G-75 has been used to separate the products from the pepsin digest by gel filtration chromatography and to desalt low density lipoproteins (LDL) by gel filtration.
Features and Benefits
- Enables rapid desalting, contaminant removal, and buffer exchange in a single step.
- Ideal for buffer exchange and cleanup of large biomolecules with a molecular weight exceeding 80,000.
- Features the highest exclusion limit of 80,000 within the Sephadex G-range.
- Classic gel filtration resin.
Legal Information
Sephadex is a registered trademark of Cytiva
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Rapid purification of fluorescent dye-labeled products in a 96-well format for high-throughput automated DNA sequencing.
K Krakowski et al.
Nucleic acids research, 23(23), 4930-4931 (1995-12-11)
Inhibition of cyclic AMP-and cyclic GMP-mediated dilations in isolated arteries by oxidized low density lipoproteins.
Galle J, et al.
Arteriosclerosis and Thrombosis, 12(2), 180-186 (1992)
Integrin alpha IIb beta 3 (platelet GPIIb-IIIa) recognizes multiple sites in fibronectin.
Bowditch, RD, et al.
Journal of biological and chemical chronicles, 266(34), 23323-23328 (1991)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G75120-500G | 04061832011363 |
| G75120-10G | 04061833642818 |
| G75120-50G | 04061833642825 |