General description
Balanced Salt solutions (BSS) are essential in cell culture as they serve as irrigating, transporting, and diluting agents to maintain the osmotic balance between intra-and extracellular spaces. They provide cells with water, inorganic ions, and energy for normal cell metabolism. BSS also provides a buffering system to maintain the medium within the optimum pH range (7.2-7.6).
Application
Hanks′ Balanced Salt has been used:
- in the preparation of stock solution for culturing mice primary hepatocytes
- to measure the mineralized nodule formation in human osteoblast cultures
- in the preparation of embryo buffer to collect zebrafish eggs
Preparation Note
Formulated to contain 9.8 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.
Other Notes
The pH is adjusted after addition of sodium bicarbonate.
Still not finding the right product?
Explore all of our products under Hanks′ Balanced Salts
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性,应注意防潮。
Related Content
Behavioral profiling of zebrafish embryos exposed to a panel of 60 water-soluble compounds
Shaukat A, et al.
Behavioural Brain Research, 228, 272-283 (2012)
Osteoblast response to porous titanium and biomimetic surface: In vitro analysis
Renata F, et al.
Materials Science and Engineering, C, 52, 194-203 (2015)
Anish Deshpande et al.
Biomolecules, 10(3) (2020-03-25)
Alpha7 nicotinic acetylcholine receptors (α7nAChRs) are interesting not only because of their physiological effects, but because this receptor requires chaperones to traffic to cell surfaces (measured by alpha-bungarotoxin [αBGT] binding). While knockout (KO) animals and antibodies that react across species
Global Trade Item Number
| SKU | GTIN |
|---|---|
| H6136-10X1L | 04061833800355 |
| H6136-2000L | 04061837459207 |