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Merck
CN

H6136

Hanks′ Balanced Salts

Without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Hanks′ Balanced Salt Solution

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217418

Key Documents

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Product Name

Hanks′ Balanced Salts, Without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Quality Level

500

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Application

Hanks′ Balanced Salt has been used:

  • in the preparation of stock solution for culturing mice primary hepatocytes
  • to measure the mineralized nodule formation in human osteoblast cultures
  • in the preparation of embryo buffer to collect zebrafish eggs

General description

Balanced Salt solutions (BSS) are essential in cell culture as they serve as irrigating, transporting, and diluting agents to maintain the osmotic balance between intra-and extracellular spaces. They provide cells with water, inorganic ions, and energy for normal cell metabolism. BSS also provides a buffering system to maintain the medium within the optimum pH range (7.2-7.6).

Other Notes

The pH is adjusted after addition of sodium bicarbonate.

Preparation Note

Formulated to contain 9.8 grams of powder per liter of medium.
Supplement with 0.35 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000427046
0000427046
0000377032
0000358395
0000351182

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Behavioral profiling of zebrafish embryos exposed to a panel of 60 water-soluble compounds
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Osteoblast response to porous titanium and biomimetic surface: In vitro analysis
Renata F, et al.
Materials Science and Engineering, C, 52, 194-203 (2015)
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

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