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Merck
CN

H8264

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

HBSS

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207

Key Documents

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Product Name

Hanks′ Balanced Salt solution, Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

shipped in

ambient

Quality Level

400

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Application

Calcium and magnesium are included in this formulation and support cell adhesion. Used to minimize cell attachment or aggregation.


Hanks’ Balanced Salt solution has been used:

  • as a rinse solution for hepatocytes
  • for hippocampi dissection
  • in corrosion testing of dog-bone specimens
  • for starvation studies in breast cancer cell line
  • as a component of dissociation solution for dorsal root ganglion
  • as a chamber solution for the patch-clamp apparatus

General description

Hanks’ Balanced Salt solution (HBSS) is a buffer that mimics biological medium and stabilizes cells. It is useful in leucocyte labeling studies. HBSS is an effective preservative for periodontal ligament cells. HBSS lacks essential inorganic ions and is useful in starvation studies.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0244
RNBL7910
RNBL6002
SLCN1542
RNBL5109

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Vladimir Benes et al.
Scientific reports, 5, 11590-11590 (2015-06-26)
microRNAs are an abundant class of small non-coding RNAs that control gene expression post-transcriptionally. Importantly, microRNA activity participates in the regulation of cellular processes and is a potentially valuable source of biomarkers in the diagnosis and prognosis of human diseases.
Hank s balanced salt solution: an alternative resuspension medium to label autologous leukocytes. Experience in inflammatory bowel disease
Martin-Comin J, et al.
Brazilian Archives of Biology and Technology, 45, 39-44 (2002)
Online oxygen monitoring using integrated inkjet-printed sensors in a liver-on-a-chip system
Moya A, et al.
Lab on a chip, 18, 2023-2035 (2018)
Emmet Galvin et al.
Medical & biological engineering & computing, 55(8), 1261-1275 (2016-10-28)
Magnesium stents are a promising candidate in the emerging field of absorbable metallic stents (AMSs). In this study, the mechanical and corrosion performance of dog-bone specimens and a specific stent design of a magnesium alloy, WE43, are assessed experimentally in
Physical removal of the midbody remnant from polarised epithelial cells using take-up by suction pressure (TUSP)
Bernabe-Rubio,M, et al
Bio-protocol, 7 (2017)
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