Skip to Content
Merck
CN

I3642

Sigma-Aldrich

Monoclonal Anti-Interleukin-1β antibody produced in mouse

clone 8516.311, purified immunoglobulin, lyophilized powder

Synonym(s):

Anti-IL-1β

Sign Into View Organizational & Contract Pricing

Select a Size

About This Item

MDL number:
MFCD00162514
UNSPSC Code:
51111800
NACRES:
NA.41

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8516.311, monoclonal

form

lyophilized powder

species reactivity

human

technique(s)

flow cytometry: suitable
immunohistochemistry: 8-25 μg/mL using human PBMC′s
neutralization: suitable
western blot: 1 μg/mL

isotype

IgG1

UniProt accession no.

P01584

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... IL1B(3553)

Related Categories

Immunogen

purifed, E. coli-derived, recombinant human IL-1β.

Application

May used in immunoblotting, immunohistochemistry, ELISA, and neutralization assays

Biochem/physiol Actions

The antibody will neutralize the biological activity of recombinant human IL-1β. It will not neutralize the activity of recombinant human IL-α, recombinant mouse IL-1α, or recombinant mouse IL-1β. When immobilized on a microplate, this antibody will capture both recombinant as well as natural human IL-1β. In immunoblotting, the antibody shows ~25% cross-reactivity with recombinant rat IL-1β and <5% cross-reactivity with recombinant mouse IL-1β and recombinant porcine IL-1β. Additionally, in immunoblotting, the antibody exhibits no cross-reactivity with recombinant mouse IL-α, recombinant human IL-1ra, recombinant mouse IL-1ra, or recombinant rat IL-1α.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Preparation Note

Monoclonal Anti-Interleukin-1(IL-1) (mouse IgG1 isotype) is purified from a mouse hybridoma utilizing Protein A affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000340371
0000323792
0000340371
0000323792
0000278609

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Melanie Genoula et al.
Frontiers in immunology, 9, 459-459 (2018-03-30)
The ability of Mycobacterium tuberculosis (Mtb) to persist in its human host relies on numerous immune evasion strategies, such as the deregulation of the lipid metabolism leading to the formation of foamy macrophages (FM). Yet, the specific host factors leading
Paul A Cordell et al.
Blood, 115(13), 2674-2681 (2010-01-21)
Factor XIII-A (FXIII-A) is present in the cytosol of platelets, megakaryocytes, monocytes, osteoblasts, and macrophages and may be released from cells by a nonclassical pathway. We observed that plasma FXIII-A levels were unchanged in thrombocytopenic mice (Bcl-x(Plt20/Plt20) and Mpl(-/-)), which
Ludmila Alekseeva et al.
BMC microbiology, 9, 33-33 (2009-02-12)
Aspergillus fumigatus, a saprophytic mould, is responsible for life-threatening, invasive pulmonary diseases in immunocompromised hosts. The role of the airway epithelium involves a complex interaction with the inhaled pathogen. Antimicrobial peptides with direct antifungal and chemotactic activities may boost antifungal
Tobias Wiedemann et al.
Gut, 61(7), 986-996 (2012-01-31)
One of the most important hormones in the human stomach is the peptide gastrin. It is mainly required for the regulation of gastric pH but is also involved in growth and differentiation of gastric epithelial cells. In Helicobacter pylori infected
Mariángeles Clauzure et al.
Immunology, 163(4), 493-511 (2021-04-10)
The impairment of the cystic fibrosis transmembrane conductance regulator (CFTR) activity induces intracellular chloride (Cl- ) accumulation. The anion Cl- , acting as a second messenger, stimulates the secretion of interleukin-1β (IL-1β), which starts an autocrine positive feedback loop. Here
View All Related Papers

Related Content

默克高品质抗体_ELISA试剂盒-默克生命科学

抗体的质量决定了整个免疫检测的成败， 是在选择抗体时首选的考虑因素。基于Sigma®、Chemicon®、Upstate®、Mil l ipore®、 Calbiochem®和Novagen®等子品牌的强强联合，默克在创新的 免疫原设计、免疫、挑选、筛选和验证等方面有着数十年的经验， 创造出许多被广泛使用的抗体，如4G10，Neun，组蛋白修饰等抗 体都是相关领域的“金标”抗体。 我们的技术和科研背景使默克成为高质量抗体的主要设计者和生产 者，而不仅仅是经销商。毋庸置疑，我们的每个小瓶中的抗体都包 含着大量的科学研究成果。

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service