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K0390

Sigma-Aldrich

Anti-Potassium Channel K2P1.1 (TWIK-1) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

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Synonym(s):
Anti-Inward rectifying potassium channel TWIK-1
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

mouse, human, rat

technique(s)

immunohistochemistry: suitable
western blot: 1:200 using rat brain lysates
western blot: 1:300 using HEK-K2P1.1 transfected cells

UniProt accession no.

O00180

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KCNK1(3775)
mouse ... Kcnk1(16525)
rat ... Kcnk1(59324)

General description

The gene K2P1.1 (two pore domain K+ channel), also referred to as KCNK1 (potassium two pore domain channel subfamily K member 1), encodes a protein that spans a length of 337-amino acids. It contains four transmembrane regions and the characteristic sequence of potassium channels in the two pore regions. The protein is found to be exclusively expressed in distal tubule. The gene is mapped to human chromosome 1q42.2.

Immunogen

synthetic peptide RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human K2P1.1 (KCNK1).

Biochem/physiol Actions

The gene KCNK1 (potassium two pore domain channel subfamily K member 1) is a double-pore potassium channel that functions in maintaining the renal potassium homeostasis. KCNK channel proteins are involved in cell volume, apoptosis, and proliferation. Alterations in these proteins have been associated with various diseases. Decreased levels of KCNK1 in human cardiac atria have been implicated in chronic atrial fibrillation. It functions as a suppressor of anchorage-independent growth in fibroblast.

Target description

K2P1.1 (also named TWIK-1 or KCNK1) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 14 members. These channels show little time or voltage dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents, which help set the membrane resting potential and cell excitation.

Physical form

Lyophilized from pphosphate buffered saline, pH 7.4, containing 1% BSA and 0.025% sodium azide.

Reconstitution

Reconstitute the lyophilized vial with 50 μL or 200 μL deionized water, depending on package size. Further dilutions should be made using a carrier protein such as BSA (1-3%).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
含少量动物源组分生物产品

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Convergent functional genomics of genome-wide association data for bipolar disorder: comprehensive identification of candidate genes, pathways and mechanisms.
Le-Niculescu H
American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics, 150B, 155-181 (2009)
Cloning and localization of a double-pore K channel, KCNK1: exclusive expression in distal nephron segments.
Orias M
The American Journal of Physiology, 273, 663-666 (1997)
Kosuke Saito et al.
Toxicological sciences : an official journal of the Society of Toxicology, 132(1), 151-161 (2013-01-08)
KCNK1, a member of the family of two-pore K(+) ion channels, is specifically induced in the livers of male mice after phenobarbital treatment. Here, we have determined the molecular mechanism of this male-specific activation of the Kcnk1 gene and characterized

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