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K3753

Krebs-Henseleit Buffer Modified

Name: Krebs-Henseleit Buffer Modified
Brand: SIGMA

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

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About This Item

UNSPSC Code:

12352207

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Properties

Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Description

General description

Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.

Application

Krebs-Henseleit Buffer Modified has been used:

to collect the rat brains for ex vivo brain slice experiments
to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)
to collect the uterine and forelimb blood vessel samples from horses

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.

Supplement with 2.1 g/L sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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