K4002
Krebs-Ringer Bicarbonate Buffer
With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution
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About This Item
NACRES:
NA.75
UNSPSC Code:
12161702
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
pH
6.1-6.7
solubility
water: 9.5 g/L, clear
storage temp.
2-8°C
General description
Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.
Application
Krebs-Ringer Bicarbonate Buffer has been used:
- as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
- in the pre-perifusion for islet viability and functionality studies
- in pituitary bioassay
Preparation Note
Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 1.26 g/L sodium bicarbonate.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Bidisha Dasgupta et al.
European journal of immunology, 38(12), 3499-3507 (2008-11-11)
When leukocytes cross endothelial cells during the inflammatory response, membrane from the recently described lateral border recycling compartment (LBRC) is selectively targeted around diapedesing leukocytes. This "targeted recycling" is critical for leukocyte transendothelial migration. Blocking homophilic PECAM interactions between leukocytes
N C Schaad et al.
Human reproduction (Oxford, England), 11(3), 561-565 (1996-03-01)
Nitric oxide is a chemical messenger which functions as a neurotransmitter or as a cytotoxic agent. Nitric oxide synthase (NOS) has been isolated from various mammalian reproductive tissues. The presence or absence of NOS in spermatozoa has not yet been
The Hypothalamic-Pituitary-Adrenal Axis Response to Stress in Mice Lacking Functional Vasopressin V1b Receptors
Stephen J L, et al.
Endocrinology, 148, 849?856-849?856 (2007)
Activation of G Protein-Coupled Receptor 43 in Adipocytes Leads to Inhibition of Lipolysis and Suppression of Plasma Free Fatty Acids
Hongfei G, et al.
Endocrinology, 149, 4519?4526-4519?4526 (2008)
Heng Xu et al.
Synapse (New York, N.Y.), 61(3), 166-175 (2006-12-08)
Previous studies established that Tyr-D-Ala-Gly-N-Me-Phe-Gly-ol (DAMGO) and (2S,4aR,6aR,7R,9S,10aS,10bR)-9-(Benzoyloxy)-2-(3-furanyl)dodecahydro-6a,10b-dimethyl-4,10-dioxo-2H-naphtho-[2,1-c]pyran-7-carboxylic acid methyl ester (herkinorin) are fully efficacious mu-agonists. Herkinorin (HERK), unlike DAMGO, does not recruit beta-arrestin and promote mu-receptor internalization, even in cells that over express beta-arrestin. We hypothesized that chronic HERK
Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| K4002-10X1L | 04061833944592 |
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