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Merck
CN

M0445

Anti-MDMX antibody, Mouse monoclonal

clone MDMX-82, purified from hybridoma cell culture

Synonym(s):

Anti-HDMX, Anti-MDM4, Anti-p53 Binding Protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00282354

Key Documents

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Product Name

Anti-MDMX antibody, Mouse monoclonal, clone MDMX-82, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MDMX-82, monoclonal

form

buffered aqueous solution

mol wt

antigen ~80 kDa

species reactivity

human

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of HEK 293T cells transfected with human MDMX

isotype

IgG1

UniProt accession no.

O15151

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... MDM4(4194)
mouse ... Mdm4(17248)
rat ... Mdm4(304798)

Related Categories

Application

Monoclonal Anti-MDMX antibody is suitable for use in immunocytochemistry, immunoprecipitation, immunoblot (approx. 80 kDa), microarray, and ELISA. The product may also be used for western blot and for assessing ectopic expression of MDMX (using a conc. of 1-2 μg/mL in total cell extract of HEK 293T cells transfected with human MDMX).

Biochem/physiol Actions

MDMX is a structural homolog of MDM2 and inhibits the tumor suppressor, p53. MDMX also prevents MDM2-mediated degradation of p53, and subsequently regulates cellular levels of p53. MDMX also modulates cell growth and survival. Thus, MDMX can be useful as a therapeutic target in cancers, especially in tumors where native p53 functions can be restored . Monoclonal Anti-MDMX antibody is specific for human MDMX.
Monoclonal Anti-MDMX recognizes human MDMX.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Double minute 4 protein or MDMX is a nuclear protein. The gene encoding it is localized on human chromosome 1q32.1.
Monoclonal Anti-MDMDX (mouse IgG1 isotype) is derived from the hybridoma MDMX-82 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. MDMX belongs to the MDM family. The MDMX/ mouse double minute 4 (MDM4) gene encode a 490 amino acid protein. MDMX comprises a putative nuclear localization signal, a really interesting new gene (RING) finger domain in the C terminal and a p53-binding domain in the N terminal. MDMX has significant structural similarity to MDM2 E3 ubiquitin ligase.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
079M4785V
0000156888
0000137826
0000137826

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Xinna Zhang et al.
Cancer research, 69(20), 7960-7968 (2009-10-08)
MdmX and Mdm2 regulate p53 tumor suppressor functions by controlling p53 transcriptional activity and/or stability in cells exposed to DNA damage. Accumulating evidence indicates that ATM-mediated phosphorylation and degradation of Mdm2 and MdmX may be the initial driving force that
MDM4 downregulates p53 transcriptional activity and response to stress during differentiation
Menendez S, et al.
Cell Cycle, 10(7), 1100-1108 (2011)
M W Jackson et al.
Molecular and cellular biology, 20(3), 1001-1007 (2000-01-11)
The p53 tumor suppressor protein is stabilized in response to cellular stress, resulting in activation of genes responsible for either cell cycle arrest or apoptosis. The cellular pathway for releasing normal cells from p53-dependent cell cycle arrest involves the Mdm2
In vitro and in silico studies of MDM2/MDMX isoforms predict Nutlin-3A sensitivity in well/de-differentiated liposarcomas
Bozzi F, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 93(11), 1-9 (2013)
Emilie A Chapeau et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(12), 3151-3156 (2017-03-08)
Inhibitors of double minute 2 protein (MDM2)-tumor protein 53 (TP53) interaction are predicted to be effective in tumors in which the TP53 gene is wild type, by preventing TP53 protein degradation. One such setting is represented by the frequent CDKN2A
View All Related Papers

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