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Merck
CN

M0894

Minimum Essential Medium Eagle

Alpha Modification, with L-glutamine and sodium pyruvate, without ribonucleosides, deoxyribonucleosides and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

MEM

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About This Item

NACRES:
NA.71
UNSPSC Code:
12352207
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form

powder

technique(s)

cell culture | mammalian: suitable

components

stable glutamine: no
Hanks’ salts (2% CO2): no
sodium pyruvate: yes
NaHCO3: no
phenol red: yes
glucose: yes
L-glutamine: yes
Earle’s salts (5% CO2): yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

Quality Level

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General description

This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins.

Preparation Note

Formulated to contain 10.1 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Alexander J Nelson et al.
Journal of lipid research, 61(2), 143-158 (2019-12-11)
Phospholipases A2 (PLA2s) catalyze hydrolysis of the sn-2 substituent from glycerophospholipids to yield a free fatty acid (i.e., arachidonic acid), which can be metabolized to pro- or anti-inflammatory eicosanoids. Macrophages modulate inflammatory responses and are affected by Ca2+-independent phospholipase A2
Jason W Ashley et al.
The Journal of biological chemistry, 291(44), 23268-23281 (2016-10-30)
Macrophages are important in innate and adaptive immunity. Macrophage participation in inflammation or tissue repair is directed by various extracellular signals and mediated by multiple intracellular pathways. Activation of group VIA phospholipase A2 (iPLA2β) causes accumulation of arachidonic acid, lysophospholipids
Tatsuki Kawasaki et al.
Development, growth & differentiation, 58(6), 530-545 (2016-06-01)
The periodontal ligament (PDL) is a connective tissue that attaches the tooth cementum to the alveolar bone and is derived from dental follicle cells (DFCs). The DFCs form fibroblasts, osteoblasts, cementoblasts, and PDL stem cells (PDLSCs). We previously reported homeobox
Shi-Yuan Sheu et al.
Cell transplantation, 29, 963689720927398-963689720927398 (2020-07-11)
Osteoporotic fracture is the main complication of osteoporosis (OP) and accounts for millions of injuries annually. Local intervention by intra-marrow injection has been a good option for preventing osteoporotic bone loss when the osteoporotic femoral fracture has been treated. In
Jun Zhang et al.
Therapeutic advances in chronic disease, 12, 2040622321995685-2040622321995685 (2021-08-31)
Previous studies reported that melatonin exerts its effect on mesenchymal stem cell (MSC) survival and differentiation into osteogenic and adipogenic lineage. In the current study we aimed to explore the effect of melatonin on osteoporosis and relevant mechanisms. Real-time qualitative

Articles

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉末培养基及盐混合物极具吸湿性,应注意防潮。

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