M7683
Anti-phospho-MAP Kinase Kinase 1/2 (pSer217/221) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Anti-phospho-MEK 1/2 (pSer217/221)
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About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
flow cytometry
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunoprecipitation (IP)
western blot
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunoprecipitation (IP)
western blot
Species reactivity:
human, rat, mouse
Citations:
10
Technique(s):
flow cytometry: 1:50 using PMA treated human peripheral blood lymphocytes and Jurkat cells.
immunocytochemistry: 1:1,000 using HeLa cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections from human breast carcinoma
immunoprecipitation (IP): 1:50
western blot: 1:1,000 using serum-treated NIH-3T3 cells
immunocytochemistry: 1:1,000 using HeLa cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections from human breast carcinoma
immunoprecipitation (IP): 1:50
western blot: 1:1,000 using serum-treated NIH-3T3 cells
Uniprot accession no.:
Product Name
Anti-phospho-MAP Kinase Kinase 1/2 (pSer217/221) antibody produced in rabbit, affinity isolated antibody, buffered aqueous glycerol solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
mol wt
antigen 45 kDa
species reactivity
human, rat, mouse
technique(s)
flow cytometry: 1:50 using PMA treated human peripheral blood lymphocytes and Jurkat cells.
immunocytochemistry: 1:1,000 using HeLa cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using tissue sections from human breast carcinoma
immunoprecipitation (IP): 1:50
western blot: 1:1,000 using serum-treated NIH-3T3 cells
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer217/pSer221)
Quality Level
Gene Information
human ... MAPK1(5594)
mouse ... Mapk1(26413)
rat ... Mapk1(116590)
Application
Anti-phospho-MAP Kinase Kinase 1/2 (MEK1/2) has a wide range of applications. The recommended working dilution for immunoblotting in serum treated NIH3T3 cells is 1:1000. For immunoprecipitation, a working dilution of 1:50 may be used. For detection by immunohistochemistry in paraffin-embedded human breast carcinoma sections, antibody dilution of 1:100 may be used. For immunocytochemistry (immunofluorescence), in HeLa cells, a working dilution of 1:1000 may be used.
Biochem/physiol Actions
Detects MEK 1/2 when phosphorylated at Ser217 and Ser221. It does not react with non-phosphorylated MEK 1/2, SEK (MKK4), MKK3, or MKK6.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
MAP Kinase Kinase (MAPKK, MEK1 and MEK2) belong to a family of enzymes that activates their substrate by dual phosphorylation at threonine and tyrosine residues. MEK1 and MEK2 have 86% homology in their catalytic domain. Activation of MEK1/2 is mediated in mitogen-stimulated cells by Raf-1 kinase. MEK1/2 then activates ERK1/2 leading to the activation of several downstream targets that contribute to cell proliferation, apoptosis and motility. In addition to ERK1/2, there may be non-catalytic effectors of MEK1/2. MEK isoforms appear to be widely expressed in the central nervous system, thymus, spleen, heart, lung, and kidney. Active forms of MEK1/2 are sufficient for the transformation of NIH3T3 cells of the differentiation of PC-12 cells.
The antibody specifically recognizes MEK1/2 (45 kDa) only when phosphorylated at Ser217 and Ser221. It reacts strongly with MEK1/2 phosphorylated only at Ser217, but less when phosphorylated only at Ser221. It shows not cross reaction with any other related family members including SEK.
The antibody specifically recognizes MEK1/2 (45 kDa) only when phosphorylated at Ser217 and Ser221. It reacts strongly with MEK1/2 phosphorylated only at Ser217, but less when phosphorylated only at Ser221. It shows not cross reaction with any other related family members including SEK.
Immunogen
synthetic phosphopeptide Ser217/221 corresponding to residues around Ser217/221 of human MEK 1/2.
Physical form
Solution in 10 mM HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol.
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Hyukjin Cha et al.
The Biochemical journal, 378(Pt 3), 857-865 (2003-12-13)
Phosphorylation-specific antibodies provide a powerful tool for analysing the regulation and activity of proteins in the MAP (mitogen-activated protein) kinase and other signalling pathways. Using synchronized cells, it was observed that phosphorylation-specific antibodies developed against the active form of MKK1/MKK2
S Cowley et al.
Cell, 77(6), 841-852 (1994-06-17)
The MAP kinase pathway is activated by a wide variety of external signals leading to cell proliferation or differentiation. However, it is not clear whether activation of this pathway is required for cellular responses or whether it is only one
S J Mansour et al.
Science (New York, N.Y.), 265(5174), 966-970 (1994-08-12)
Mitogen-activated protein (MAP) kinase kinase (MAPKK) activates MAP kinase in a signal transduction pathway that mediates cellular responses to growth and differentiation factors. Oncogenes such as ras, src, raf, and mos have been proposed to transform cells by prolonging the
Jan Pinkas et al.
Cancer research, 62(16), 4781-4790 (2002-08-17)
Activation of the mitogen-activated protein/extracellular signal-regulated kinase kinase (MEK)-mitogen-activated protein kinase (MAPK) pathway is a frequent event in tumorigenesis, and analysis of human breast carcinomas demonstrates that 25-50% of these tumors express elevated levels of activated MAPK1/2. However, a direct
M Raman et al.
Oncogene, 26(22), 3100-3112 (2007-05-15)
Mitogen-activated protein kinases (MAPKs) regulate diverse cellular programs including embryogenesis, proliferation, differentiation and apoptosis based on cues derived from the cell surface and the metabolic state and environment of the cell. In mammals, there are more than a dozen MAPK
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