usage
sufficient for 100 colorimetric tests
detection method
colorimetric
relevant disease(s)
hematological disorder
storage temp.
−20°C
General description
Glucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high.
Application
Glucose-6-phosphate assay kit has been used in:
- glucose 6-phosphate (G6P) assay
- quantification of glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P)
- metabolite analysis
Suitable for quantifying Glucose-6-Phosphate in a variety of samples.
Biochem/physiol Actions
G6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. G6P is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the G6P present. Typical sensitivities of detection are between 1-30 nmoles.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
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