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Merck
CN

MAK017

Lactose Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
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usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric, fluorometric

relevant disease(s)

cancer; neonatal diseases; pediatric diseases

storage temp.

−20°C

General description

Lactose is a dissacharide formed by the condensation of one galactose and one glucose molecule. Lactose is the major sugar in the milk of most species, typically present between 2-8%. The enzyme lactase hydrolyzes lactose to its constituent monosaccharides. In neonates, glucose released via the action of lactase is a major energy source.

Application

Lactose Assay Kit has been used to measure lactose content in milk.
Suitable for the direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.)

Biochem/physiol Actions

In this assay, lactose concentration is determined by an enzymatic assay, in which lactose is converted to galactose and glucose. The galactose is subsequently oxidized, resulting in a colorimetric (570 nm)/ fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the lactose initially present. This kit is suitable for use with various biological samples and has a linear range of detection between 0.2-1.0 nmole lactose for the fluorometric assay and 2-10 nmoles lactose for the colorimetric assay.


pictograms

Health hazard

signalword

Danger

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 3

hcodes

Hazard Classifications

Resp. Sens. 1

Regulatory Information

常规特殊物品

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