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MAK099

Sigma-Aldrich

Glutamate Dehydrogenase (GDH) Activity Assay Kit

sufficient for 100 colorimetric tests

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usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer; gastrointestinal diseases

storage temp.

−20°C

Gene Information

human ... GLUD1(2746) , GLUD2(2747)
mouse ... GLUD1(14661)
rat ... GLUD1(24399)

Related Categories

General description

Glutamate Dehydrogenase (GDH) is a mitochondrial enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and serves as a key link between anabolic and catabolic pathways. In mammals, GDH is subject to allosteric regulation and has high activity in liver, kidney, brain, and pancreas. GDH activity in serum can be used to differentiate between liver diseases due to liver inflammation, which do not show elevated serum GDH activity, and diseases that result in hepatocyte necrosis, which results in elevated serum GDH.

Application

Glutamate Dehydrogenase (GDH) Activity Assay Kit has been used to determine the enzymatic activity of glutamate dehydrogenase.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for detecting glutamate dehydrogenase (GDH) activity in serum or tissue and cell extracts.

Principle

GDH activity is determined by a coupled enzyme assay in which glutamate is consumed by GDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the GDH activity present. One unit of GDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.6 at 37 °C.

Storage Class Code

10 - Combustible liquids

Regulatory Information

常规特殊物品

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L-leucine stimulates glutamate dehydrogenase activity and glutamate synthesis by regulating mTORC1/SIRT4 pathway in pig liver.
Wang T, et al.
Animal Nutrition (2017)
Ian Monroe et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 228, 29-34 (2018-11-06)
Fathead minnows, Pimephales promelas, are found throughout the continental United States in waters in which salinity can change with tides and temperatures vary seasonally. They have been used extensively in studies of environmental toxicology and are commercially important. In a
Inactivation of Lsd1 triggers senescence in trophoblast stem cells by induction of Sirt4.
Castex J, et al.
Cell Death & Disease, 8(2), e2631-e2631 (2017)
The glutamate dehydrogenase pathway and its roles in cell and tissue biology in health and disease.
Plaitakis A, et al.
Biology, 6(1), 11-11 (2017)
Is liver enzyme release really associated with cell necrosis induced by oxidant stress?.
Contreras-Zentella M L and Hernandez-Mu?oz R
Oxidative Medicine and Cellular Longevity, 2016 (2016)
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