MAK208
GPDH Activity Assay Kit
Sufficient for 100 Colorimetric tests
Synonym(s):
Glycerol 3-Phosphate Dehydrogenase Activity Assay Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12352200
detection method
colorimetric
storage temp.
−20°C
Gene Information
human ... GPD1(2819), GPD2(2820)
mouse ... GPD1(14555), GPD2(14571)
rat ... GPD1(60666), GPD2(25062)
General description
Glycerol 3-phosphate dehydrogenase (GPDH; EC 1.1.1.8) catalyzes the reversible conversion of dihydroxyacetone phosphate and NADH to glycerol-3-phosphate and NAD+. It forms a glycerol phosphate shuttle and facilitates the transfer of reduced equivalents across the mitochondria to the cytosol. GPDH is important for both carbohydrate and lipid metabolism. Mutations in GPDH gene result in transient infantile hypertriglyceridemia.
Application
GPDH (glyceraldhyde-3-phoshate dehydrogenase) activity assay kit has been used to measure the serum and hepatic concentrations of GPDH in rabbit.
Suitable for the detection of Glycerol-3-Phosphate Dehydrogenase Activity in tissue and cell culture samples.
Biochem/physiol Actions
The Glycerol 3-Phosphate Dehydrogenase Activity Colorimetric Assay Kit provides a simple and sensitive procedure for measuring GPDH activity in a variety of tissues and cells. GPDH activity is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the enzymatic activity present. One unit of GPDH is the amount of enzyme required to generate 1.0 μmole of NADH per minute at pH 8 at 37 °C.
Kit Components Only
Product No.
Description
- GPDH Assay Buffer
- GPDH Substrate
- GPDH Probe
- NADH Standard
- GPDH Positive Control
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class
8A - Combustible corrosive hazardous materials
Regulatory Information
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Regulation of adenine nucleotide translocase and glycerol 3-phosphate dehydrogenase expression by thyroid hormones in different rat tissues.
DUMMLER K, et al.
The Biochemical Journal, 317(3), 913-918 (1996)
A compound heterozygous mutation in GPD1 causes hepatomegaly, steatohepatitis, and hypertriglyceridemia.
Joshi M, et al.
European Journal of Human Genetics, 22(10), 1229-1229 (2014)
B. Elmahdi, MA Al-Omair, AA El-Bessoumy and SM El-Bahr.
Thomson I S I
International Journal of Pharmaceutics, 12(4), 429-434 (2016)
Structure of glycerol-3-phosphate dehydrogenase (GPD1) from Saccharomyces cerevisiae at 2.45 ? resolution.
Alarcon D A, et al.
Acta Crystallographica Section F, Structural Biology and Crystallization Communications, 68(11), 1279-1283 (2012)
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