Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP⁺ /NADPH has applications in research pertaining to energy transformation and the redox state of cells or tissue.

Sensitive and accurate - Detection limit of 0.01 mM and linearity up to 1 mM NADP⁺ /NADPH in 96 well plate assay.
Convenient – The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 minutes. Room temperature assay.
High-throughput – Can be readily automated as a high-throughput 96 well plate assay for thousands of samples per day.

Suitable for measuring NADP+ /NADPH concentrations and ratio in cell or tissue extracts.

Simple, direct and automation-ready procedures for measuring NADP⁺ /NADPH concentration are very desirable. This NADP⁺ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex = 530 nm/λem = 585 nm, is proportional to the NADP⁺ /NADPH concentration in the sample. This assay is highly specific for NADP⁺/ NADPH and with minimal interference (<1%) by NAD⁺ /NADH. This assay is a convenient, direct method to measure NADP⁺ /NADPH concentrations and ratio in cell or tissue extracts.

The NADP⁺ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lex = 530 nm/lem = 585 nm, is proportional to the NADP⁺ /NADPH concentration in the sample. This assay is highly specific for NADP⁺ / NADPH and with minimal interference (<1%) by NAD⁺ /NADH. This assay is a convenient, direct method to measure NADP⁺ /NADPH concentrations and ratio in cell or tissue extracts.