MAK312
NADP/NADPH Assay Kit
sufficient for 100 fluorometric tests
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About This Item
NACRES:
NA.84
UNSPSC Code:
12352200
detection method
fluorometric
relevant disease(s)
cancer
storage temp.
−20°C
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General description
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and the redox state of cells or tissue.
Application
Suitable for measuring NADP+ /NADPH concentrations and ratio in cell or tissue extracts.
Biochem/physiol Actions
Simple, direct and automation-ready procedures for measuring NADP+ /NADPH concentration are very desirable. This NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex = 530 nm/λem = 585 nm, is proportional to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+/ NADPH and with minimal interference (<1%) by NAD+ /NADH. This assay is a convenient, direct method to measure NADP+ /NADPH concentrations and ratio in cell or tissue extracts.
The NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lex = 530 nm/lem = 585 nm, is proportional to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ / NADPH and with minimal interference (<1%) by NAD+ /NADH. This assay is a convenient, direct method to measure NADP+ /NADPH concentrations and ratio in cell or tissue extracts.
Features and Benefits
- Sensitive and accurate - Detection limit of 0.01 μM and linearity up to 1 μM NADP+/NADPH in 96 well plate assay.
- Convenient - The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 minutes. Room temperature assay.
- High-throughput - Can be readily automated as a high-throughput 96 well plate assay for thousands of samples per day.
Sensitive and accurate - Detection limit of 0.01 mM and linearity up to 1 mM NADP+ /NADPH in 96 well plate assay.
Convenient – The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 minutes. Room temperature assay.
High-throughput – Can be readily automated as a high-throughput 96 well plate assay for thousands of samples per day.
Convenient – The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 minutes. Room temperature assay.
High-throughput – Can be readily automated as a high-throughput 96 well plate assay for thousands of samples per day.
signalword
Danger
hcodes
Hazard Classifications
Met. Corr. 1 - Resp. Sens. 1
Storage Class
8B - Non-combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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