N0913
Anti-Neurocan antibody, Mouse monoclonal
clone 650.24, purified from hybridoma cell culture
Synonym(s):
Anti-CSPG3, Anti-Chondroitin Sulfate Proteogycan 3
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About This Item
Conjugate:
unconjugated
Clone:
650.24, monoclonal
Application:
ELISA (i), ICC, IHC (f), WB
Citations:
11
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
650.24, monoclonal
form
buffered aqueous solution
mol wt
antigen 260 kDa (additional band at 160 kDa)
species reactivity
rat
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunocytochemistry: suitable, immunohistochemistry (frozen sections): suitable, indirect ELISA: suitable, western blot: 0.1-0.2 μg/mL using neonatal rat brain treated with chondroitinase.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Ncan(58982)
General description
Monoclonal Anti-Neurocan (mouse IgG1 isotype) is derived from the hybridoma 650.24 produced by the fusion of mouse myeloma cells (P3X cells) and splenocytes from BALB/c mice immunized with embryonic rat brain proteoglycans. Neurocan is a chondroitin sulfate proteoglycan (known also as CSPG3). It accounts for 20−30% of chondroitin sulfate proteoglycans in early postnatal rat brain. The mouse neurocan cDNA encodes for a 1,257-amino acid protein with a 22-amino acid signal peptide followed by an immunoglobulin-like domain and repeating motifs characteristic of the hyaluronic acid-binding region of aggregating proteoglycans. Neurocan is found in large amounts in glial scar, a primarily astrocytic structure formed after an injury in the central nervous system (CNS).
Neurocan is a chondroitin sulfate proteoglycan (knownalso as CSPG3) thought to be involved in the modulation of cell adhesion and migration. It accounts for 20-30% of chondroitin sulfate proteoglycans in early postnatal rat brain.
Neurocan is a proteoglycan which is expressed in the nervous tissue.
Immunogen
embryonic rat brain proteoglycans.
Application
Anti-Neurocan antibody, Mouse monoclonal has been used in:
- western blotting
- immunofluorescence
- enzyme-linked immunosorbent assay (ELISA)
- immunocytochemistry
- immunohistochemistry
Biochem/physiol Actions
Neurocan can inhibit the growth of axons. In presence of central nervous system injuries, it also takes part in tissue repair and neural network reconstruction.
Neurocan participates in the modulation of cell adhesion and migration. It exerts a negative effect on growing cerebellar axons.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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