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NA0300S

Sigma-Aldrich

GenElute HP Plasmid Maxiprep Kit

greener alternative

sufficient for 4 purifications

Synonym(s):
GenElute HP Plasmid Maxiprep Kit
NACRES:
NA.52

Quality Level

usage

sufficient for 4 purifications

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative category

Aligned

storage temp.

15-25°C

General description

The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute HP Plasmid Maxiprep Kit has been used to isolate plasmid DNA from Escherichia coli.

Features and Benefits

  • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
  • Up to 1.2 mg of high-copy plasmid DNA
  • Flexibility of a vacuum or spin format
  • Contains fewer plastic components than other high speed kits, reducing the amount of waste
  • No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Certificate of Analysis

Certificate of Origin

Lisa A Santry et al.
Molecular therapy. Methods & clinical development, 9, 181-191 (2018-03-21)
Newcastle disease virus (NDV) is a single-stranded, negative-sense RNA virus in the Paramyxoviridae family. Although primarily an avian pathogen, NDV is a potent oncolytic virus that has been shown to be safe and effective in a variety of preclinical cancer
Development of novel plasmid vectors and a promoter trap system in Francisella tularensis compatible with the pFLN10 based plasmids
David A Rasko
Plasmid, 58(2) (2007)
David A Rasko et al.
Plasmid, 58(2), 159-166 (2007-04-27)
Francisella tularensis is a category A bioterror pathogen which in some cases can cause a severe and fatal human infection. Very few virulence factors are known in this species due to the difficulty in working with it as well as
Ilaria Mogno et al.
Genome research, 23(11), 1908-1915 (2013-08-08)
Gene promoters typically contain multiple transcription factor binding sites (TFBSs), which may vary in affinity for their cognate transcription factors (TFs). One major challenge in studying cis-regulation is to understand how TFBS variants affect gene expression. We studied the in

Protocols

GenElute™ HP Plasmid Maxiprep Procedure

Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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