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Merck
CN

P4874

Anti-PADI4 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-PAD4, Anti-PADI5, Anti-PDI4, Anti-Peptidyl Arginine Deiminase Type IV, Anti-Peptidyl Arginine Deiminase V

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About This Item

MDL number:
MFCD09039201
UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 67 kDa

species reactivity

human

technique(s)

immunoprecipitation (IP): 2-4 μL using DMSO-treated HL60 cells
western blot: 1:500-1:1,000 using DMSO-treated HL60 cells

UniProt accession no.

Q9UM07

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PADI4(23569)

General description

PAD4 (also designated PADI4 or PAD type IV), one of the members of the peptidyl arginine deiminases (PADIs).
Peptidyl arginine deiminase 4 (PADI4) protein consists of 663 amino acids. PADI4 gene is localized to human chromosome 1p36. It is expressed in hematopoietic tissues, such as spleen, thymus, peripheral blood leucocytes, fetal liver and bone marrow.

Immunogen

synthetic peptide corresponding to amino acids 91-107 of human PADI4, conjugated to KLH via a C-terminal added cysteine residue. The immunizing peptide is specific to human PADI4.

Application

Anti-PADI4 (N-terminal) antibody produced in rabbit has been used in immunoblotting,immunoprecipitation and immunofluorescence.

Biochem/physiol Actions

PAD4 is recruited to the pS2 promoter (an estrogen responsive gene) following hormone induction when the gene is transcriptionally downregulated.
Peptidyl arginine deiminase 4 (PADI4) is responsible for the post-translational conversion of peptidylarginine to citrulline, in the presence of calcium ions. In individuals with rheumatoid arthritis (RA), this gene is expressed in hematological cells and synovial tissues, and variant in this gene is linked with susceptibility to RA. The expression of this protein is linked with DNA hypermethylation in acute promyelocytic leukemia (APL).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
078M4884V
078M4884V
106K4780

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Histone deimination antagonizes arginine methylation
Cuthbert GL, et al.
Cell, 118(5), 545-553 (2004)
A novel PAD4/SOX4/PU.1 signaling pathway is involved in the committed differentiation of acute promyelocytic leukemia cells into granulocytic cells
Guanhua Song
Oncotarget (2016)
Localization of peptidylarginine deiminase 4 (PADI4) and citrullinated protein in synovial tissue of rheumatoid arthritis.
Chang X
Rheumatology (Oxford, England) (2005)
Functional haplotypes of PADI4, encoding citrullinating enzyme peptidylarginine deiminase 4, are associated with rheumatoid arthritis.
Suzuki A
Nature Genetics (2003)
A novel PAD4/SOX4/PU.1 signaling pathway is involved in the committed differentiation of acute promyelocytic leukemia cells into granulocytic cells.
Song G
Oncotarget (2016)
View All Related Papers

Related Content

Data Sheet - P4874

Instructions

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