P7059
Phosphate Buffered Saline
10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology
Synonym(s):
Phosphate buffered saline, PBS
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About This Item
Product Name
Phosphate Buffered Saline, 10× PBS for Western blots and IP
grade
Molecular Biology
Quality Level
sterility
0.2 μm filtered
form
liquid
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
pH
7.2-7.6(1x solution)
storage temp.
room temp
General description
Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.
Application
Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.
Preparation Note
10x PBS contains:
- 30mM sodium phosphate
- 1.5 M sodium chloride
- 10.5 mM potassium phosphate
Dilute 10x PBS to a 1x working solution using molecular biology grade water.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.
Johan E Karlsson et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(4), 705-714 (2010-01-15)
Membrane-bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney
Jennifer L S Sporty et al.
Analytical chemistry, 82(15), 6593-6600 (2010-07-14)
A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring nerve agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 microL serum
Shahani Noor et al.
Brain, behavior, and immunity, 61, 80-95 (2016-12-25)
A growing body of evidence indicates that prenatal alcohol exposure (PAE) may predispose individuals to secondary medical disabilities later in life. Animal models of PAE reveal neuroimmune sequelae such as elevated brain astrocyte and microglial activation with corresponding region-specific changes
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P7059-1L | 04061834394297 |