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R1280

Monoclonal Anti-RPA/p34 antibody produced in mouse

~2 mg/mL, clone 9H8, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-REPA2, Anti-RPA2, Anti-RPA32, Anti-Replication protein A2; 32kDa

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD03845826

Key Documents

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Product Name

Monoclonal Anti-RPA/p34 antibody produced in mouse, ~2 mg/mL, clone 9H8, purified immunoglobulin, buffered aqueous solution

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9H8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~34 kDa

species reactivity

human, monkey

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.1-0.2 μg/mL using HeLa total cell extract

isotype

IgG1

UniProt accession no.

P15927

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... RPA2(6118)

Application

The antibody may be used in:
  • immunoblotting (~34 kDa)
  • immunocytochemistry
  • immunoprecipitation

Biochem/physiol Actions

Replication protein A (RPA) complex is essential for processes like DNA replication, recombination, and DNA repair pathways. The 32-kDa subunit of human RPA is hyper-phosphorylated in its N-terminus upon cellular DNA damage. This phosphorylation causes a change in RPA conformation that down-regulates its activity in DNA replication but does not affect DNA repair processes. Thus the role of RPA phosphorylation in the cellular response to DNA damage is to help regulate DNA metabolism and promote DNA repair.The RPA complex, once bound to single stranded DNA, recruits the ATR (ataxia-telangiectasia mutated and Rad3-related)-ATRIP (ATR-interacting protein) protein kinase complex that is crucial for the cellular response to replication stress and DNA damage and for ATR-mediated Chk1 activation in human cells. Furthermore, the association of this complex to the single stranded DNA causes the phosphorylation of RAD17 protein that is bound to DNA. Yeast strains deficient in RPA are defective in recruiting Ddc2 (yeast homologue of ATRIP) and thus down stream proteins are not phosphorylated. Replication protein A binds with the ssDNA with great affinity and interacts specifically with multiple proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-RPA/p34 (mouse IgG1 isotype) is derived from the hybridoma 9H8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human RPA/p34. Replication protein A Replication protein A (RPA) complex is a single-stranded DNA-binding protein and it is made up of 70-, 32- and 14-kDa subunits.

Immunogen

purified human RPA/p34.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
047K4824

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Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
Zou L and Elledge S J
Science (New York, N.Y.), 300(5625), 1542-1548 (2003)
W Q Qiu et al.
The Journal of biological chemistry, 271(14), 8443-8451 (1996-04-05)
Progressive cerebral deposition of the amyloid beta-peptide (Abeta) is an early and constant feature of Alzheimer's disease. Abeta is derived by proteolysis from the beta-amyloid precursor protein. beta-Amyloid precursor protein processing and the generation of Abeta have been extensively characterized
Replication protein A phosphorylation and the cellular response to DNA damage
Binz S K, et al.
DNA Repair, 3(8-9), 1015-1024 (2004)
M K Kenny et al.
The Journal of biological chemistry, 265(13), 7693-7700 (1990-05-05)
Human single-stranded DNA binding protein (human SSB) is a multisubunit protein containing polypeptides of 70, 34, and 11 kDa that is required for SV40 DNA replication in vitro. In this report we identify the functions of the SSB and its

Related Content

Data Sheet - R1280

Instructions

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