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About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Product Name
Monoclonal Anti-RFP antibody produced in mouse, clone GT1433, affinity isolated antibody
biological source
mouse
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
GT1433, monoclonal
form
buffered aqueous solution
mol wt
27kDa
concentration
0.98mg/mL
technique(s)
immunoprecipitation (IP): suitable
western blot: 5000-20000
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Application
Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:5000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. Monoclonal Anti-RFP antibody produced in mouse has been used in chromatin immunoprecipitation (ChIP) assay.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
General description
Red fluorescent protein (RFP) is obtained from the Discosoma coral. It can be used as a fluorescent probe for optical microscopy and acts as a biological marker for biomolecule imaging. RFPs exhibit fluorescence emission beyond 57 0nm. They have lower light-scattering, low photo-toxicity and less autofluorescence interference of cells. RFPs are extremely useful for whole body and deep tissue imaging.
Immunogen
Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of RFP.
Other Notes
Purification: Affinity purified by Protein G
Physical form
Phosphate-buffered saline, no preservative added.
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Misregulation of a DDHD Domain-containing Lipase Causes Mitochondrial Dysfunction in Yeast*
Pradeep Kumar Yadav and Ram Rajasekharan
The Journal of Biological Chemistry (2016)
Guide to Red Fluorescent Proteins and Biosensors for Flow Cytometry
Kiryl D
Methods in Cell Biology (2011)
A monomeric red fluorescent protein
Robert E. Campbell
Proceedings of the National Academy of Sciences of the USA (2002)
DNA mimics of red fluorescent proteins (RFP) based on G-quadruplex-confined synthetic RFP chromophores
Nucleic Acids Research, 45(18) (2017)
Kayla Davis et al.
Life science alliance, 6(1) (2022-10-28)
Mitochondrial transport relies on a motor-adaptor complex containing Miro1, a mitochondrial outer membrane protein with two GTPase domains, and TRAK1/2, kinesin-1, and dynein. Using a peroxisome-directed Miro1, we quantified the ability of GTPase mutations to influence the peroxisomal recruitment of
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