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SAB4200094

Anti-PKM2 (isoform M1) antibody produced in rabbit

Name: Anti-PKM2 (isoform M1) antibody produced in rabbit
Brand: SIGMA

~1.5 mg/mL, affinity isolated antibody

Enhanced Validation

Synonym(s):

Anti-CTHBP, Anti-OIP3 (OPA-interacting protein 3), Anti-PK3, Anti-PKM, Anti-Pyruvate Kinase, MUSCLE (isoform M1), Anti-TCB, Anti-THBP1 (thyroid hormone-binding protein, cytosolic)

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About This Item

NACRES:

NA.44

UNSPSC Code:

12352203

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ICC, IHC, IP, WB

Citations:

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

human, rat, mouse

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1.5 mg/mL

technique(s)

immunocytochemistry: 5-10 μg/mL using HeLa cells, immunohistochemistry: 20-30 μg/mL using formalin-fixed paraffin embedded human colon, immunoprecipitation (IP): 2-4 μg using rat brain extract (S2 fraction), western blot: 1-2 μg/mL using mouse brain extract (S2 fraction)

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PKM2(5315)

Description

General description

Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis and has four known isoforms, namely, L, R, M1 and M2. The M2 isoform has been linked to cancer metastasis. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues.

Application

Anti-PKM2 (isoform M1) antibody produced in rabbit has been used in:

immunoblotting
immunoprecipitation
immunohistochemistry
immunocytochemistry

Biochem/physiol Actions

Anti-PKM2 (isoform M1) antibody is specific for human, mouse and rat PKM2 (Isoform M1)/PKM1. In immunoblotting, detection of the PKM2 (Isoform M1) /PKM1 band is specifically inhibited by the immunizing peptide.

Pyruvate kinase (PK) is a key enzyme in the glycolytic pathway. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect, and reduced ability to form tumors in mouse xenografts. Phosphorylation of the M2 isoform at Tyr¹⁰⁵inhibits its activity and is common in human cancers, suggesting that Tyr¹⁰⁵ is a critical metabolic switch in cancer cells that promotes tumorigenesis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)