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SAB4200369

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse

clone R2.5254.2, purified from hybridoma cell culture

Synonym(s):

Anti-CD112, Anti-HVEB, Anti-PRR2, Anti-PVRL2, Anti-PVRR2, Anti-Poliovirus receptor-related 2 (herpesvirus entry mediator B)

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
R2.5254.2, monoclonal
Application:
flow cytometry
immunocytochemistry
indirect ELISA
western blot
Species reactivity:
rat, human, monkey, mouse
Citations:
8
Technique(s):
flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells
immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells
indirect ELISA: suitable
western blot: suitable

Key Documents

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Product Name

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse, clone R2.5254.2, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

R2.5254.2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~72 kDa

species reactivity

rat, human, monkey, mouse

concentration

~1.0 mg/mL

technique(s)

flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells
immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells
indirect ELISA: suitable
western blot: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... NECTIN2(5819), PVRL2(5819), PVRL2(308417)
mouse ... Nectin2(19294)

Related Categories

Application

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunocytochemistry
  • flow cytometry

Biochem/physiol Actions

Nectins act as key elements of adherens junctions and facilitate their formation. Nectin-2 possesses anti-viral property against herpes simplex virus (HSV)-2 infection. In mice, loss of nectin-2 affects sperm production and male-specific infertility. Aberrations in the gene has been associated with the pathogenesis of peripheral T-cell lymphoma (PTCL). Serum levels of nectin-2 can be used as a diagnostic and prognostic marker for colorectal cancer (CRC).
The antibody has been reported to block cell adhesion and herpes virus entry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-Nectin-2/PVRL2 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with 3T3 cells expressing human nectin-2. Nectin-2 is found to be widely expressed in human tissues, including hematopoietic cells.
Nectin-2, also known as poliovirus receptor related protein 2 gene (PRR2), is encoded by the gene mapped to human chromosome 19q13.2. The encoded protein belongs to the nectin protein family and is characterized with an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain.

Immunogen

3T3 cells expressing human Nectin-2.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
0000167337
0000146846
0000146846
0000167337
121M4763V

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Viral entry mechanisms: cellular and viral mediators of herpes simplex virus entry.
Akhtar J and Shukla D
FEBS Journal, 276, 7228-7236 (2009)
PVRL2 is translocated to the TRA@ locus in t(14;19)(q11;q13)-positive peripheral T-cell lymphomas.
Almire C
Genes Chromosomes Cancer, 46, 1011-1018 (2007)
Serum nectin-2 levels are diagnostic and prognostic in patients with colorectal carcinoma.
Karabulut M
Clinical And Translational Oncology, 18, 160-171 (2016)
Sequencing of 42kb of the APO E-C2 gene cluster reveals a new gene: PEREC1.
Dna Sequence, 9, 89-100 (1998)
The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice.
Barron MJ
Human Molecular Genetics, 17, 3509-3520 (2008)
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