SAB4200369
Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse
clone R2.5254.2, purified from hybridoma cell culture
别名:
Anti-CD112, Anti-HVEB, Anti-PRR2, Anti-PVRL2, Anti-PVRR2, Anti-Poliovirus receptor-related 2 (herpesvirus entry mediator B)
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
R2.5254.2, monoclonal
Application:
flow cytometry
immunocytochemistry
indirect ELISA
western blot
immunocytochemistry
indirect ELISA
western blot
Species reactivity:
rat, human, monkey, mouse
Citations:
8
Technique(s):
flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells
immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells
indirect ELISA: suitable
western blot: suitable
immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells
indirect ELISA: suitable
western blot: suitable
产品名称
Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse, clone R2.5254.2, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
R2.5254.2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~72 kDa
species reactivity
rat, human, monkey, mouse
concentration
~1.0 mg/mL
technique(s)
flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells
immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells
indirect ELISA: suitable
western blot: suitable
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NECTIN2(5819), PVRL2(5819), PVRL2(308417)
mouse ... Nectin2(19294)
Application
Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
- flow cytometry
Biochem/physiol Actions
Nectins act as key elements of adherens junctions and facilitate their formation. Nectin-2 possesses anti-viral property against herpes simplex virus (HSV)-2 infection. In mice, loss of nectin-2 affects sperm production and male-specific infertility. Aberrations in the gene has been associated with the pathogenesis of peripheral T-cell lymphoma (PTCL). Serum levels of nectin-2 can be used as a diagnostic and prognostic marker for colorectal cancer (CRC).
The antibody has been reported to block cell adhesion and herpes virus entry.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Nectin-2/PVRL2 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with 3T3 cells expressing human nectin-2. Nectin-2 is found to be widely expressed in human tissues, including hematopoietic cells.
Nectin-2, also known as poliovirus receptor related protein 2 gene (PRR2), is encoded by the gene mapped to human chromosome 19q13.2. The encoded protein belongs to the nectin protein family and is characterized with an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain.
Immunogen
3T3 cells expressing human Nectin-2.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Viral entry mechanisms: cellular and viral mediators of herpes simplex virus entry.
Akhtar J and Shukla D
FEBS Journal, 276, 7228-7236 (2009)
PVRL2 is translocated to the TRA@ locus in t(14;19)(q11;q13)-positive peripheral T-cell lymphomas.
Almire C
Genes Chromosomes Cancer, 46, 1011-1018 (2007)
Sequencing of 42kb of the APO E-C2 gene cluster reveals a new gene: PEREC1.
Dna Sequence, 9, 89-100 (1998)
Serum nectin-2 levels are diagnostic and prognostic in patients with colorectal carcinoma.
Karabulut M
Clinical And Translational Oncology, 18, 160-171 (2016)
The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice.
Barron MJ
Human Molecular Genetics, 17, 3509-3520 (2008)
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