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Merck
CN

SAB4200543

Anti-Calbindin-D-28K antibody, Mouse monoclonal

clone CB-955, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-(28kD), Monoclonal Anti-D-28K, Monoclonal Anti-D-dependent calcium-binding protein, Monoclonal Anti-RTVL-H protein, Monoclonal Anti-avian-type, Monoclonal Anti-calbindin 1, Monoclonal Anti-vitamin

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About This Item

NACRES:
NA.44
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-Calbindin-D-28K antibody, Mouse monoclonal, clone CB-955, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CB-955, monoclonal

form

buffered aqueous solution

mol wt

antigen ~28 kDa

species reactivity

mouse, human, rat

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin embedded rat cerebellum.
western blot: 2-4 μg/mL using MDBK extract.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... CALB1(793)

Related Categories

Application

  • Anti-Calbindin-D-28K antibody, Mouse monoclonal has been used in:
  • western blotting
  • enzyme-linked immunosorbent assay (ELISA){31
  • immunohistochemistry
  • immunohistochemical procedure
  • indirect immunofluorescence

Biochem/physiol Actions

Calbindin D-28k might play a vital role as an intracellular Ca2+ buffer. It has an ability to protect nerve cells from calcium-mediated neurotoxicity. The encoded protein functions as a potent neuroanatomical marker which can be used to selectively visualize certain neurons and pathways in the central nervous system and peripheral nervous system. Mutations in the gene has been observed in patients with Parkinson′s disease (PD).
Calbindin-D (28K) functions as a mobile or partly immobilized Ca2+ -buffer with medium kinetics and affinity buffer function. Second, it functions in buffered Ca2+- diffusion-transport function. Finally, it interacts with target proteins in its apo-and Ca2+-loaded form-sensor- like function. Over-expression of this protein stimulates neurite outgrowth in dopaminergic neuronal cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-Calbindin-D-28K antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CB-955 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified bovine kidney calbindin-D-28K.
Calbindin-D-28K (CALB1) is encoded by the gene mapped to human chromosome 8q21.3-q22.1. CALB1 belongs to the EF hand superfamily of Ca2+ binding proteins (CBPs). The encoded protein contains EF hand motif involved in the specific and reversible binding of Ca2+. It is highly expressed in specific regions of central nervous system such as cerebellar Purkinje cells and hippocampal granule cells.

Immunogen

purified bovine kidney calbindin-D-28K. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000508814
0000508814
0000455882
0000455882
0000362515

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Calbindin-D28K-containing neurons in animal models of neurodegeneration: possible protection from excitotoxicity
Iacopino A
Brain Research Molecular Brain Research, 13, 251-261 (1992)
Overexpression of calbindin-D28K induces neurite outgrowth in dopaminergic neuronal cells via activation of p38 MAPK
Choi WS, et al.
Biochemical and biophysical research communications, 287(3), 656-661 (2001)
Central role of the proximal tubular alpha Klotho/FGF receptor complex in FGF23-regulated phosphate and vitamin D metabolism
Takeshita A, et al.
Scientific reports, 8(1), 6917-6917 (2018)
Calbindin D-28k and parvalbumin in the rat nervous system
Celio MR
Neuroscience, 35, 375-475 (1990)
A splice site variant in INPP5E causes diffuse cystic renal dysplasia and hepatic fibrosis in dogs
Dillard KJ, et al.
Testing, 13(9) (2018)
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