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UNSPSC Code:
12352203
NACRES:
NA.41
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biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

RPOL-1, monoclonal

form

buffered aqueous solution

mol wt

antigen ~250 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoblotting: 1-2 μg/mL using nuclear extracts of human HeLa cells.

isotype

IgG1

UniProt accession no.

P24928

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... POLR2A(5430)
mouse ... Polr2a(20020)
rat ... Polr2a(363633)

General description

Monoclonal Anti-POLR2A (mouse IgG1 isotype) is derived from the hybridoma RPOL-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Polymerase (RNA) II (DNA directed) polypeptide A (POLR2A) is the largest subunit of RNA polymerase II. It is mapped on the human chromosome at 17p13.1.

Specificity

Monoclonal Anti-POLR2A recognizes human POLR2A.

Immunogen

Synthetic peptide corresponding to the N-terminal region of human POLR2A, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, bovine, dog and pig.

Application

Monoclonal Anti-POLR2A antibody produced in mouse may be used in immunoblotting.

Biochem/physiol Actions

Polymerase (RNA) II (DNA directed) polypeptide A (POLR2A) plays a role in the synthesis of messenger RNA in eukaryotes. It contains a carboxy-terminal domain composed of heptapeptide repeats that are essential for polymerase activity. POLR2A in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
033M4757V
033N4757V
033M4757

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