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Merck
CN

SAB4200716

Anti-Vimentin antibody, Mouse monoclonal

clone VIM-13.2, purified from hybridoma cell culture

Synonym(s):

Anti-F5H288

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-Vimentin antibody, Mouse monoclonal, clone VIM-13.2, purified from hybridoma cell culture

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

VIM-13.2, monoclonal

form

buffered aqueous solution

mol wt

~58 kDa

species reactivity

mouse, bovine, canine, rat, monkey, human, chicken

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract
immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells
immunohistochemistry: suitable

isotype

IgM

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

bovine ... Vim(280955)
chicken ... Vim(420519)
dog ... Vim(477991)
human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)
rhesus monkey ... Vim(705289)

Related Categories

Application

Anti-Vimentin antibody, Mouse monoclonal has been used in:
  • western blotting
  • indirect immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells.

Immunogen

human foreskin fibroblast extract

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0000475646
0000475646
0000420465
0000420465
0000397787

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The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy
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Gadau SD
Neuroscience Letters, 687, 111-118 (2018)
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Development (Cambridge, England), 145(4) (2018-01-24)
Stem cells (SCs) drive mammary development, giving rise postnatally to an epithelial bilayer composed of luminal and basal myoepithelial cells. Dysregulation of SCs is thought to be at the origin of certain breast cancers; however, the molecular identity of SCs
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