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Merck
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SAB4301134

Anti-6-His antibody produced in rabbit

affinity isolated antibody

Synonym(s):

6 His epitope tag, Anti His Antibody Sigma, Anti His Antibody Sigma - Anti-6×His antibody produced in rabbit

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
polyclonal
Technique(s):
western blot: 1:500-1:2000 (Cell Lysate)
Application:
western blot
Citations:
29

Key Documents

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Product Name

Anti-6-His antibody produced in rabbit, affinity isolated antibody

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

46 kDa

concentration

0.5 mg/mL

technique(s)

western blot: 1:500-1:2000 (Cell Lysate)

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Application

Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.
The Anti-6-His antibody has been used in western blotting.

Biochem/physiol Actions

The antibody detects transfected proteins contanining 6×His tag.
The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

General description

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.

Immunogen

Synthetic peptide: 18XHis conjugated to KLH

Physical form

Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
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Certificates of Analysis (COA)

Lot/Batch Number
2331135533
2184335533
2251335533
2131435533
2111335533

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Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation
Jiang Y, et al.
Nature Cell Biology, 17(9), 1158-1158 (2015)
The FLAG? peptide, a versatile fusion tag for the purification of recombinant proteins.
Einhauer A and Jungbauer A
Journal of Biochemical and Biophysical Methods, 49(1-3), 455-465 (2001)
A novel USP9X substrate TTK contributes to tumorigenesis in non-small-cell lung cancer.
Chen X, et al.
Theranostics, 8(9), 2348-2348 (2018)
O-GlcNAcylation of fumarase maintains tumour growth under glucose deficiency
Wang T, et al.
Nature Cell Biology, 19(7), 833-833 (2017)
Yun Chen et al.
Nucleic acids research, 46(7), 3643-3656 (2018-03-27)
TARS and TARS2 encode cytoplasmic and mitochondrial threonyl-tRNA synthetases (ThrRSs) in mammals, respectively. Interestingly, in higher eukaryotes, a third gene, TARSL2, encodes a ThrRS-like protein (ThrRS-L), which is highly homologous to cytoplasmic ThrRS but with a different N-terminal extension (N-extension).
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