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Merck
CN

SAB5300513

Monoclonal Anti-BECN1 antibody produced in mouse

clone 2A4, ascites fluid

Synonym(s):

ATG6, BECN1, VPS30, beclin1

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
2A4, monoclonal
Application:
direct ELISA
flow cytometry
immunohistochemistry
western blot
Species reactivity:
human
Citations:
2
Technique(s):
direct ELISA: 1:10,000
flow cytometry: 1:200-1:400
immunohistochemistry: 1:200-1:1,000
western blot: 1:500-1:2,000
Uniprot accession no.:
Q14457

Key Documents

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Product Name

Monoclonal Anti-BECN1 antibody produced in mouse, clone 2A4, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2A4, monoclonal

mol wt

60 kDa

species reactivity

human

technique(s)

direct ELISA: 1:10,000
flow cytometry: 1:200-1:400
immunohistochemistry: 1:200-1:1,000
western blot: 1:500-1:2,000

isotype

IgG1

NCBI accession no.

NM_003766.3

UniProt accession no.

Q14457

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... BECN1(8678)

Related Categories

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified recombinant fragment of human BECN1 expressed in E.coli.
Mouse monoclonal antibody raised against BECN1

Physical form

Ascitic fluid containing 0.03% sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
PM1104185

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Tahereh Kashkoulinejad-Kouhi et al.
Oncology reports, 45(2), 665-679 (2021-01-09)
Cisplatin treatment confers the relative resistance to MCF-7 cells as compared to other breast cancer cell lines. One principal reason is that chemotherapeutic agents induce autophagy in these cells to inhibit apoptosis. Binding immunoglobulin protein (BiP), a master regulator of unfolded
Jia Wang et al.
Molecular medicine reports, 12(3), 4210-4218 (2015-06-18)
The aim of the present study was to determine whether liraglutide (LRG), a long acting glucagon-like peptide 1 analogue, exerted a protective effect on free fatty acid (FFA)‑treated pancreatic β‑cells via activating autophagy. INS‑1 insulinoma pancreatic islet cell lines were

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