SAE0150
Chondroitinase ABC from Proteus vulgaris
recombinant, low endotoxin, aqueous solution, ≥100 U/ml, 50-250 units/mg protein, BSA free
Synonym(s):
Ch′ase ABC, Chondroitin ABC Lyase
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Biological source:
bacterial (Proteus vulgaris)
biological source
bacterial (Proteus vulgaris)
Quality Segment
conjugate
(Glucosaminoglycan)
specific activity
50-250 units/mg protein
shipped in
dry ice
storage temp.
−70°C
General description
Chondroitinase ABC is a lyase with specificity toward chondroitin sulfates and dermatan sulfates - with some minor activity toward hyaluronan. This recombinant version only contains the endolytic chondroitinase, meaning it will not digest the non-reducing ends of proteoglycan substrates. It targets β-(1,4)-linkages between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid. The product of digestion is a mixture of Δ 4-unsaturated tetra- and di-saccharides.
Application
- Degradation of Chondroitin sulfate-containing tissue such as extracellular matrices and cartilage
- Neural regeneration research
- Eliminative degradation of all forms of Chondroitin sulfate: A, B and C
- Quantitation of glucosaminoglycans (mucopolysaccharides)
Features and Benefits
- Low Endotoxin
- BSA Free
- Recombinant
- Endolytic component only, does not contain exolytic enzyme.
- Compatible with chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate
Preparation Note
Store the product at -70 °C. When stored unopened at -70 °C, the buffer is stable for at least two years.
Other Notes
1 unit will liberate 1.0 μmole of unsaturated disaccharides per min at pH 8.0 at 37 °C, using chondroitin sulfate from shark cartilage (C4384) as substrate.
Non active Ingredients: Hepes-Sodium salt, NaCl, Trehalose, Imidazole, Glycerol, Water
This product is for R&D use only. Not for drug, household, or other uses. Please consult the SafetyData Sheet for information regarding hazards and safe handling practices.
pH optimum: pH 8.0 (chondroitin sulfate)pH 6.8 (hyaluronic acid)
Temperature optimum: 37 °C
Activator: 0.05 M acetate
Inhibitors: 1 mM Zn2+ Ni2+, Fe2+ and Cu2+
Temperature optimum: 37 °C
Activator: 0.05 M acetate
Inhibitors: 1 mM Zn2+ Ni2+, Fe2+ and Cu2+
Storage Class
10 - Combustible liquids
wgk
WGK 2
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