SBR00051
Trizma® hydrochloride solution
pH 8.5, DNase and Microbial DNA free, 10 mM
Synonym(s):
Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
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About This Item
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
NACRES:
NA.25
UNSPSC Code:
12161700
Beilstein/REAXYS Number:
3675235
MDL number:
Product Name
Trizma® hydrochloride solution, pH 8.5, DNase and Microbial DNA free, 10 mM
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
concentration
10 mM
pH
8.5
useful pH range
7.0-9.0
pKa (25 °C)
8.1
storage temp.
2-8°C
Quality Level
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Application
Trizma® hydrochloride solution can be used:
- As an elution buffer after PCR cleanup
- As an elution buffer after DNA extraction
General description
10 mM Trizma® hydrochloride (pH 8.5) is a widely used buffer in molecular biology applications such as nucleic acid extraction and purification. No mixing or pH adjustment is necessary. Guaranteed accuracy for this buffer is ± 0.2 pH units at 25°C.
Other Notes
Prepared with BioUltra Trizma Base for molecular biology in 18 megaohm UV irradiated water. pH-adjusted and 0.2 μm filtered. The buffer was tested by 35 cycles of PCR for prokaryotic and eukaryotic DNA contamination. To keep the buffer contamination-free, it is recommended to use gloves and barrier-tip pipettes for dispensing. Between uses, keep the bottle closed and refrigerated, away from areas where DNA samples and PCR products may be stored.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Preparation Note
Allow the buffer to reach room temperature before use.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
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