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Merck
CN

T1819

Trizma® hydrochloride solution

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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About This Item

NACRES:
NA.25
UNSPSC Code:
12161700

Key Documents

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Product Name

Trizma® hydrochloride solution, pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

grade

BioPerformance Certified
Molecular Biology

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

Quality Level

200

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Application

Trizma® hydrochloride solution has been used:
  • as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii
  • in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies
  • for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies

General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0. It is applicable in electrophoretic studies and capillary electrochromatography.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000493664
0000493664
0000469305
0000469301
0000469305

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A review of acellular immersion tests on bioactive glasses-influence of medium on ion release and apatite formation
Nommeots-Nomm A, et al.
International Journal of Applied Glass Science, 11(3) (11)
(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39) (5)
Junya Yamagish et al.
Methods in molecular biology (Clifton, N.J.), 1201, 193-205 (2014-11-13)
Parasites change their transcriptional systems in different developmental stages and in response to environmental changes. To investigate the molecular mechanisms that underlie transcriptional regulation, it is essential to identify the exact positions of the transcriptional start sites (TSSs) and characterize
Production and characterisation of biodegradable PLA nanoparticles loaded with thymol to improve its antimicrobial effect
Marcet I, et al.
Journal of Food Engineering, 239 (239)
Kaichun Zhu et al.
Nature protocols, 1(6), 3088-3093 (2007-04-05)
This protocol describes a streamlined method of plasmid DNA extraction by continual thermal lysis, a modification of the basic boiling lysis technique, to simplify the processing of large volumes of Escherichia coli cultures. Fermented bacteria are harvested using a hollow

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