T2145
Tyrode′s Salts
Powder, without sodium bicarbonate, suitable for cell culture
Synonym(s):
Tyrode′s Buffer, Tyrode′s Solution, Tyrode′s buffer
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
Tyrode′s Salts, Without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
General description
Tyrode′s Salts play an important role in tissue culture. A salt solution′s basic function is to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
Application
Tyrode′s Salts has been used:
- for seeding cardiomyocytes culture for optical based phenotyping and imaging studies
- to incubate 3T3-L1 pre-adipocytes in calcium intracellular assays
- as a medium for the isolation and culturing of myofiber extensor digitorum longus (EDL) muscle
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
Supplement with 1.0 g/L sodium bicarbonate.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Theodora Pavlidou et al.
Stem cells international, 2019, 5980465-5980465 (2019-06-30)
The regeneration of the muscle tissue relies on the capacity of the satellite stem cell (SC) population to exit quiescence, divide asymmetrically, proliferate, and differentiate. In age-related muscle atrophy (sarcopenia) and several dystrophies, regeneration cannot compensate for the loss of
Yu-Fen Chang et al.
PloS one, 12(4), e0174181-e0174181 (2017-04-06)
Identification of drug induced electrical instability of the heart curtails development, and introduction, of potentially proarrhythmic drugs. This problem usually requires complimentary contact based approaches such as patch-clamp electrophysiology combined with field stimulation electrodes to observe and control the cell.
Anna Marianne Weijler et al.
Frontiers in immunology, 11, 586856-586856 (2020-12-29)
Humoral immunity is a major barrier limiting long-term outcome after organ transplantation. Especially, the production of antibodies directed against donor HLA/MHC antigens (i.e. donor-specific antibodies (DSA)) leading to antibody-mediated rejection (ABMR) is considered to be a major factor negatively affecting
James F X Jones et al.
Experimental physiology, 88(3), 329-334 (2003-04-30)
The cranial venae cavae of the rat heart are composed of cardiac muscle. We tested whether the vagus nerve has an inotropic action on these blood vessels. Stimulation of right or left vagal fibres (n = 7 animals) produced a
Felipe Mora-Bermúdez et al.
eLife, 3 (2014-07-06)
Mitotic spindle orientation is crucial for symmetric vs asymmetric cell division and depends on astral microtubules. Here, we show that distinct subpopulations of astral microtubules exist, which have differential functions in regulating spindle orientation and division symmetry. Specifically, in polarized
Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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