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T5326

Sigma-Aldrich

Anti-γ-Tubulin antibody, Mouse monoclonal

enhanced validation

clone GTU-88, purified from hybridoma cell culture

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MDL number:
MFCD00677366
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified antibody (purified from hybridoma cell culture)

antibody product type

primary antibodies

clone

GTU-88, monoclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

rat, hamster, chicken, human, bovine, canine, Xenopus, mouse

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

immunocytochemistry: 1-2 μg/mL using HeLa cells
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of chicken fibroblasts

isotype

IgG1

UniProt accession no.

P23258

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBG1(7283)
mouse ... Tubg1(103733)
rat ... Tubg1(252921)

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General description

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Specificity

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

Immunogen

synthetic γ-tubulin peptide, conjugated to KLH

Application

Monoclonal Anti-γ-Tubulin antibody produced in mouse has been used in following studies:
  • Western blot analysis
  • Immunohistochemistry
  • Immunocytology

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Clinical and cellular features in patients with primary autosomal recessive microcephaly and a novel CDK5RAP2 mutation.
Issa L
Orphanet Journal of Rare Diseases, 8:59 (2013)
Cilia localization is essential for in vivo functions of the Joubert syndrome protein Arl13b/Scorpion.
Duldulao NA and S, Sun Z
Development, 136(23), 4033-4042 (2009)
Characterization of stress response in human retinal epithelial cells.
Giansanti V
Journal of Cellular and Molecular Medicine, 17(1), 103-115 (2013)
The gamma-tubulin gene family in humans.
Wise DO
Genomics, 67(2), 164-170 (2000)
Causes and consequences of gray matter heterotopia.
Watrin F
CNS Neuroscience & Therapeutics, 21(2), 112-122 (2015)
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