T9285
Tris-EDTA buffer solution
100X concentrate for Northern and Southern blotting
Synonym(s):
Tris-EDTA wash buffer, TE buffer solution
grade
Molecular Biology
Quality Level
sterility
0.2 μm filtered
form
liquid
pH
7.8-8.2 (1 × in water)
application(s)
sample preparation
foreign activity
DNase, RNase, none detected
storage temp.
room temp
SMILES string
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key
VLEIUWBSEKKKFX-UHFFFAOYSA-N
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General description
Application
- isolate, resuspend, or store DNA and RNA for PCR or qPCR
- gel electrophoresis, including Northern and Southern blotting
- bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
- antigen retrieval for immunohistochemistry
Preparation Note
Other Notes
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?
When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.
What is the pH and color of Product T9285, Tris-EDTA buffer solution?
The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.
What is the shelf life of Product T9285, Tris-EDTA buffer solution?
This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.
What does 'for molecular biology' mean?
The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.
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