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T9784

Tricine

Name: Tricine
Brand: SIGMA

BioXtra, pH 4.0-6.0 (1 M in H₂O), ≥99% (titration)

Synonym(s):

N-[Tris(hydroxymethyl)methyl]glycine

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About This Item

Linear Formula:

(HOCH₂)₃CNHCH₂CO₂H

CAS Number:

5704-04-1

Molecular Weight:

179.17

UNSPSC Code:

12161700

NACRES:

NA.25

PubChem Substance ID:

24900603

EC Number:

227-193-6

Beilstein/REAXYS Number:

1937804

MDL number:

MFCD00004277

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product line

BioXtra

Quality Level

400

assay

≥99% (titration)

form

powder

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

4.0-6.0 (1 M in H₂O)

useful pH range

7.4-8.8

pKa (25 °C)

8.1

solubility

H₂O: 1 M, clear, colorless

anion traces

chloride (Cl^-): ≤0.1%, sulfate (SO₄^2-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH₄⁺: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%

absorption

≤0.05 at 280 in H₂O at 1 M, ≤0.1 at 260 in H₂O at 1 M

application(s)

diagnostic assay manufacturing

SMILES string

OCC(CO)(CO)NCC(O)=O

InChI

1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)

InChI key

SEQKRHFRPICQDD-UHFFFAOYSA-N

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Related Categories

Biochemicals

Biological Buffers

High-Purity Biochemicals and Reagents

General description

The name Tricine is derived from "tris" and "glycine". Tricine and other good buffers serve as efficient scavengers of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is widely used as an electrophoresis buffer and for the resuspension of cell pellets. Due to its lower negative charge compared to glycine, it migrates faster. It has a high ionic strength which leads to more ion movement and less protein movement. This aids the separation of low molecular weight proteins in lower percent acrylamide gels. Tricine is also used as a trailing ion that permits the resolution of small proteins at lower acrylamide concentrations than in glycine-sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) systems.

Application

Buffer component for separation of low molecular weight peptides.

Tricine has been used:

in a polymerase chain reaction (PCR) buffer used in the single-cell collection
as a component of lactotroph feeding medium for culturing lactotrophs
to prepare luciferase assay reagent for luciferase activity assay in firefly Photonis pyralis and as a component in Triton/glycylglycine lysis buffer

Storage Class

13 - Non Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Encyclopedia of Dairy Sciences (2011)

Detection of Heteroplasmic Variants in the Mitochondrial Genome through Massive Parallel Sequencing.

Joke Mertens et al.

Bio-protocol, 9(13), e3283-e3283 (2019-07-05)

Detecting heteroplasmies in the mitochondrial DNA (mtDNA) has been a challenge for many years. In the past, Sanger sequencing was the main option to perform this analysis, however, this method could not detect low frequency heteroplasmies. Massive Parallel Sequencing (MPS)

Analysis of gene promoter regulation by tumor suppressor genes.

Kumaravel Somasundaram et al.

Methods in molecular biology (Clifton, N.J.), 223, 101-116 (2003-06-05)

High-resolution membrane capacitance measurements for the study of exocytosis and endocytosis.

Boštjan Rituper et al.

Nature protocols, 8(6), 1169-1183 (2013-05-25)

In order to understand exocytosis and endocytosis, it is necessary to study these processes directly. An elegant way to do this is by measuring plasma membrane capacitance (C(m)), a parameter proportional to cell surface area, the fluctuations of which are

Variations on a theme: changes to electrophoretic separations that can make a difference.

Thierry Rabilloud

Journal of proteomics, 73(8), 1562-1572 (2010-04-17)

Electrophoretic separations of proteins are widely used in proteomic analyses, and rely heavily on SDS electrophoresis. This mode of separation is almost exclusively used when a single dimension separation is performed, and generally represents the second dimension of two-dimensional separations.

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Global Trade Item Number

SKU	GTIN
T9784-250G	04061837396052
T9784-100G	04061837391569
T9784-25G	04061833625125
T9784-1KG	04061826720448

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