U4758
Anti-U2AF65 antibody, Mouse monoclonal
clone MC3, purified from hybridoma cell culture
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About This Item
Conjugate:
unconjugated
Clone:
MC3, monoclonal
Application:
ARR, ELISA (i), EM, ICC, IHC, IP, WB
Citations:
52
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MC3, monoclonal
form
buffered aqueous solution
mol wt
antigen ~65 kDa
species reactivity
human, rat, Xenopus, mouse
packaging
antibody small pack of 25 μL
technique(s)
electron microscopy: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.1-0.2 μg/mL using total cell extract from HeLa cells
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... U2AF2(11338)
mouse ... U2af2(22185)
rat ... U2af2(308335)
General description
Monoclonal Anti-U2AF65 (mouse IgG2b isotype) is derived from the MC3 hybridoma produced by the fusion of mouse myeloma cells (Ag8.653 cells) and splenocytes from BALB/c mice immunized with recombinant human U2AF65. U1, U2, U4/U6, and U5 are small nuclear riboprotein (snRNPs) that are major subunits of spliceosomes. Spliceosomes also contain non snRNPs proteins such as alternative splicing factor/splicing factor 2 (ASF/SF2), 35 kDa spliceosomal component (SC-35) and U2 snRNP auxiliary splicing factor (U2AF). U2 snRNP auxiliary factor 65 kDa (U2AF65) is a splicing factor which has three C-terminal RNA recognition motifs (RRMs) and an N-terminal arginine/serine-rich (RS) domain. This gene is mapped to human chromosome 19q13.42. Splicing factor U2AF65kDa subunit is a non-snRNP protein component of spliceosome complexes.
Immunogen
recombinant human U2AF65.
Application
Monoclonal Anti-U2AF65 antibody produced in mouse has been used in:
- RNA immunoprecipitation
- immunoblotting
- chromatin immunoprecipitation (ChIP)
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
- immunocytochemistry
- immunoelectron microscopy
Biochem/physiol Actions
U2 snRNP auxiliary factor 65 kDa (U2AF65)/U2AF2 helps to supply the small nuclear ribonucleoprotein (snRNP) U2, which is subunit of the spliceosome. It can induce the exclusion of alternative exons. U2AF65 primarily regulates the splicing of pre-mRNA. The serine-rich (RS) repeats at the N-terminal region act as a signalling factor for nuclear localization. (U2AF65) and (U2AF35) are components of the splicing factor U2AF that transports between the cytoplasm and the nucleus.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
低风险生物材料
常规特殊物品
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Find documentation for the products that you have recently purchased in the Document Library.
Splicing inhibition of U2AF65 leads to alternative exon skipping
Cho S, et al.
Proceedings of the National Academy of Sciences of the USA, 112(32), 9926-9931 (2015)
In vitro iCLIP-based modeling uncovers how the splicing factor U2AF2 relies on regulation by cofactors
Sutandy FXR, et al.
Genome Research, 28(5), 699-713 (2018)
Gene expression profiling of U2AF2 dependent RNA-protein interactions during CD4+ T cell activation
Whisenant TC
Genomics Data, 11(3), 77-80 (2017)
Splicing-coupled 3' end formation requires a terminal splice acceptor site, but not intron excision.
Lee Davidson et al.
Nucleic acids research, 41(14), 7101-7114 (2013-05-30)
Splicing of human pre-mRNA is reciprocally coupled to 3' end formation by terminal exon definition, which occurs co-transcriptionally. It is required for the final maturation of most human pre-mRNAs and is therefore important to understand. We have used several strategies
Xinjun Ji et al.
Nucleic acids research, 44(5), 2283-2297 (2016-02-21)
Alternative splicing (AS) is a robust generator of mammalian transcriptome complexity. Splice site specification is controlled by interactions of cis-acting determinants on a transcript with specific RNA binding proteins. These interactions are frequently localized to the intronic U-rich polypyrimidine tracts
Global Trade Item Number
| SKU | GTIN |
|---|---|
| U4758-100UL | 04061838038050 |
| U4758-200UL | 04061838107824 |
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